Genetically engineered chimeric silk-silver binding proteins.
Genetically engineered chimeric silk-silver binding proteins.
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DOI：
10.1002/adfm.201100249
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发表时间：
2011-08-09
影响因子：
19
通讯作者：
Kaplan, David L.
中科院分区：
文献类型：
--
作者：
Currie, Heather A.;Deschaume, Olivier;Naik, Rajesh R.;Perry, Carole C.;Kaplan, David L.
复合材料或杂化材料在自然界中很常见，是通过离子在有机模板上的浓缩和随后的成核形成的，有机模板通常是基于蛋白质的。例如，含钙盐在骨骼、牙齿和内耳中的沉积，以及趋磁细菌中的氧化铁结构。生物有机体使用有限数量的金属离子，主要是钙和铁，少量的锶和钡。利用其他离子生成复合材料的能力提供了新材料性能的可能性。含有银的新材料在抗菌功能方面将是有用的。因此，在本研究中，报道了一条获得这种功能化生物材料的新途径。基因工程融合蛋白是通过将组合生物扫描鉴定的银结合短肽的核苷酸掺入到蜘蛛丝的共同序列中而产生的，所得到的丝-银结合蛋白从硝酸银溶液中核化银离子，而丝蛋白提供了一种稳定的模板材料，可以加工成薄膜、纤维和三维支架。丝膜抑制了琼脂平板上和液体培养中革兰氏阳性和革兰氏阴性微生物的生长，从而突出了这些嵌合材料系统作为抗菌生物医学涂层的潜力。
Composite or hybrid materials are commonly found in Nature, formed through the concentration and subsequent nucleation of ions upon organic templates that are most often protein based. Examples include the deposition of calcium containing salts in bone, teeth and the inner ear and iron oxide structures in magnetotactic bacteria. Biological organisms use a limited number of metal ions, the principal ones being calcium and iron, with lesser amounts of strontium, and barium. The ability to utilize other ions to generate composites offers the possibility of new material properties. New materials incorporating silver would be useful in the context of antimicrobial functions. Therefore, in the present study, a new route to such functionalized biomaterials is reported. Genetically engineered fusion proteins are created by the incorporation of nucleotides corresponding to short silver binding peptides identified by a combinatorial biopanning process into the consensus sequence of silk from the spider,Nephila clavipes.The resulting chimeric silk–silver binding proteins nucleated Ag ions from a solution of silver nitrate while the silk protein provided a stable template material which could be processed into films, fibers, and three‐dimensional scaffolds. The silk films inhibited microbial growth of both Gram‐positive and Gram‐negative microrganisms on agar plates and in liquid culture, thus highlighting the potential of these chimeric material systems as antimicrobial biomedical coatings.
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影响因子：
56.9
作者：
Guerette, PA;Ginzinger, DG;Gosline, JM
通讯作者：
Gosline, JM
影响因子：
4.4
作者：
Pal, Sukdeb;Tak, Yu Kyung;Song, Joon Myong
通讯作者：
Song, Joon Myong
影响因子：
4.2
作者：
Currie, Heather A.;Perry, Carole C.
通讯作者：
Perry, Carole C.
影响因子：
19
作者：
Naik, RR;Jones, SE;Stone, MO
通讯作者：
Stone, MO
影响因子：
6.2
作者：
Bini, Elisabetta;Foo, Cheryl Wong Po;Kaplan, David L.
通讯作者：
Kaplan, David L.