SV40 associated miRNAs are not detectable in mesotheliomas.
SV40 associated miRNAs are not detectable in mesotheliomas.
复制标题
DOI：
10.1038/sj.bjc.6605848
复制
发表时间：
2010-09-07
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
猿猴病毒-40（SV 40）是一种DNA肿瘤病毒，通过污染的脊髓灰质炎病毒疫苗引入人群，其在间皮瘤中的作用受到广泛争议。基于PCR的检测受到质疑，因为假阳性可能是由于与相关病毒的交叉反应或实验室污染。医学研究所建议开发更敏感和更具体的测试来解决这一争议。我们已经表征了高度灵敏的基于RT-PCR的检测方法，该方法对SV 40编码的microRNA（miRNAs）具有特异性，作为当前检测方法的替代方法。使用这种敏感和特异的检测方法，我们无法鉴定SV 40 miRNA在人类恶性胸膜间皮瘤（MM）样本中的表达。我们的工作表明，SV 40 miRNA不太可能有助于间皮瘤肿瘤发生，但与目前相对非特异性的检测方法相比，突出了这种方法的价值。
Simian virus-40 (SV40) is a DNA tumour virus that was introduced into the human population with contaminated poliovirus vaccine, and its role in mesothelioma is widely debated. PCR based testing has been called into question, as false positives can be because of cross-reactivity with related viruses, or to laboratory contamination. The Institute of Medicine has recommended the development of more sensitive and specific tests to resolve this controversy. We have characterized highly sensitive RT–PCR based assays that are specific for SV40-encoded microRNAs (miRNAs), as an alternative to current testing methods. Using this sensitive and specific detection method, we were unable to identify SV40 miRNA expression in human malignant pleural mesothelioma (MM) samples. Our work indicates that SV40 miRNAs are not likely to contribute to mesothelioma tumourogenesis, but highlights the value of this approach when compared with the relatively unspecific current testing methods.
登录
查看更多内容
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
5.4
作者：
Seo, G. J.;Fink, L. H. L.;Sullivan, C. S.
通讯作者：
Sullivan, C. S.
DOI：
10.3181/00379727-105-26128
发表时间：
1960-01-01
影响因子：
--
作者：
SWEET, BH;HILLEMAN, MR
通讯作者：
HILLEMAN, MR
影响因子：
64.8
作者：
Sullivan, CS;Grundhoff, AT;Ganem, D
通讯作者：
Ganem, D
影响因子：
64.5
作者：
BOTCHAN, M;TOPP, W;SAMBROOK, J
通讯作者：
SAMBROOK, J