Conditioned medium from a rat ureteric bud cell line in combination with bFGF induces complete differentiation of isolated metanephric mesenchyme.
Conditioned medium from a rat ureteric bud cell line in combination with bFGF induces complete differentiation of isolated metanephric mesenchyme.
复制标题
来自大鼠输尿管芽细胞系的条件培养基与 bFGF 组合可诱导分离的后肾间质的完全分化。
作者：
I. Karavanova;L. Dove;J. Resau;A. Perantoni
后肾间充质的分化由来自上皮输尿管芽的诱导信号触发。作为这种诱导的结果，大部分后肾间充质转化为肾单位的上皮。我们已经开发和特点的外植体培养系统，其中后肾间充质可以生长和完全分化的诱导组织的情况下，在体外。当分离的13 dpc大鼠后肾间充质培养在无血清条件培养基中从大鼠输尿管芽细胞系（RUB 1）在bFGF和TGF α的存在下，他们被诱导分化为肾单位上皮细胞和肾小球样结构。从形态学和分子生物学两方面证实了肾单位的分化类型，并证实了肾单位分化的发育变化。在培养1-9天后，通过整体包埋和组织切片原位杂交分析外植体中刷状缘抗原以及肾分化的分子标记物Wt 1、Lim 1、Hgf和c-met、c-ret、Shh、Wnt 4、Wnt 7 b和Wnt 11的表达模式。通过RT-PCR和北方印迹杂交证实了分泌的图案分子Bmp 7和Wnt 7 b的表达，但不表达Shh或Wnt 11。我们的培养系统有助于检查这些和其他信号分子的相关性，需要肾单位分化。
Differentiation of metanephric mesenchyme is triggered by an inductive signal(s) from the epithelial ureteric bud. As a result of this induction, most of the metanephric mesenchyme converts into epithelium of a nephron. We have developed and characterized an explant culture system, in which metanephric mesenchyme can grow and completely differentiate in vitro in the absence of an inductive tissue. When separated 13 dpc rat metanephric mesenchymes were cultured in serum-free conditioned medium from a rat ureteric bud cell line (RUB1) in the presence of bFGF and TGFalpha, they were induced to differentiate into nephron epithelia and glomeruli-like structures. The nephric type of differentiation was confirmed by both morphological and molecular criteria and paralleled the developmental changes of nephron differentiation in vivo. Expression patterns of brush-border antigen as well as molecular markers of kidney differentiation Wt1, Lim1, Hgf and c-met, c-ret, Shh, Wnt4, Wnt7b, and Wnt11 were analyzed in explants by whole mount and tissue section in situ hybridization following 1-9 days in culture. The expression of secreted patterning molecules Bmp7 and Wnt7b, but not Shh or Wnt11, were demonstrated by RT-PCR and northern blot hybridization with RNA from the RUB1 cells. Our culture system lends itself to examining the relevance of these and other signaling molecules required for nephron differentiation.
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影响因子：
56.9
作者：
I. Tsarfaty;S. Rong;J. Resau;S. Rulong;P. P. D. Silva-P.;G. Woude
通讯作者：
I. Tsarfaty;S. Rong;J. Resau;S. Rulong;P. P. D. Silva-P.;G. Woude
影响因子：
10.5
作者：
PELLETIER, J;SCHALLING, M;HOUSMAN, D
通讯作者：
HOUSMAN, D
影响因子：
4.6
作者：
V. Pachnis;B. Mankoo;F. Costantini
通讯作者：
V. Pachnis;B. Mankoo;F. Costantini
影响因子：
10.5
作者：
LUO, G;HOFMANN, C;KARSENTY, G
通讯作者：
KARSENTY, G
后肾胚基在体外分化为集合系统和肾单位上皮。
DOI：
10.1242/dev.121.10.3207
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Qiao,J;Cohen,D;Herzlinger,D
通讯作者：
Herzlinger,D