Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development.
Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development.
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DOI：
10.3791/62568
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发表时间：
2021-06-09
期刊：
影响因子：
--
通讯作者：
West AE
中科院分区：
文献类型：
--
作者：
Chan U;Gautam D;West AE
Neurons undergo dynamic changes in their structure and function during brain development to form appropriate connections with other cells. The rodent cerebellum is an ideal system to track the development and morphogenesis of a single cell type, the cerebellar granule neuron (CGN), across time. Here, in vivo electroporation of granule neuron progenitors in the developing mouse cerebellum was employed to sparsely label cells for subsequent morphological analyses. The efficacy of this technique is demonstrated in its ability to showcase key developmental stages of CGN maturation, with a specific focus on the formation of dendritic claws, which are specialized structures where these cells receive the majority of their synaptic inputs. In addition to providing snapshots of CGN synaptic structures throughout cerebellar development, this technique can be adapted to genetically manipulate granule neurons in a cell-autonomous manner to study the role of any gene of interest and its effect on CGN morphology, claw development, and synaptogenesis.
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影响因子：
4.6
作者：
Dhar M;Hantman AW;Nishiyama H
通讯作者：
Nishiyama H
影响因子：
56.9
作者：
Konishi, Y;Stegm端ller, J;Bonni, A
通讯作者：
Bonni, A
影响因子：
64.8
作者：
BenArie, N;Bellen, HJ;Zoghbi, HY
通讯作者：
Zoghbi, HY
影响因子：
56.9
作者：
Shalizi, A;Gaudillière, B;Bonni, A
通讯作者：
Bonni, A
染色质重塑使活性基因失活并调节神经编码。
DOI：
10.1126/science.aad4225
发表时间：
2016-07-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Yang Y;Yamada T;Hill KK;Hemberg M;Reddy NC;Cho HY;Guthrie AN;Oldenborg A;Heiney SA;Ohmae S;Medina JF;Holy TE;Bonni A
通讯作者：
Bonni A