Gene expression profiles in human gastric cancer: expression of maspin correlates with lymph node metastasis.
Gene expression profiles in human gastric cancer: expression of maspin correlates with lymph node metastasis.
复制标题
人类胃癌中的基因表达谱：Maspin的表达与淋巴结转移相关。
DOI：
10.1038/sj.bjc.6602429
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发表时间：
2005-03-28
影响因子：
8.8
通讯作者：
Mori, K
中科院分区：
文献类型：
--
作者：
Terashima, M;Maesawa, C;Oyama, K;Ohtani, S;Akiyama, Y;Ogasawara, S;Takagane, A;Saito, K;Masuda, T;Kanzaki, N;Matsuyama, S;Hoshino, Y;Kogure, M;Gotoh, M;Shirane, M;Mori, K
为了寻找调控胃癌进展和转移的候选基因，我们利用DNA微阵列技术研究了基因表达谱。从21名胃癌患者的肿瘤组织和邻近的正常组织中获得，然后通过Gene Chip® Human U95 Av 2阵列检测其基因表达谱，该阵列包括12 000个人类基因和EST序列。在肿瘤组织中，共有25个基因上调，两个基因下调至少四倍。在进一步分析淋巴结转移时，maspin、癌胚抗原和非特异性交叉反应抗原在有淋巴结转移的胃癌中的表达水平均显著高于无淋巴结转移的胃癌。无肠上皮化生的正常胃上皮细胞中未观察到Maspin表达。相比之下，maspin在85个肿瘤组织中的74个中表达。maspin阳性肿瘤染色的发生率与淋巴结转移有显著相关性。这些结果表明maspin在胃癌的肿瘤转移中具有潜在的作用。
To seek for a candidate gene that would regulate tumour progression and metastasis in gastric cancer, we investigated gene expression profiles by using DNA microarray. Tumour tissue and adjacent normal tissue were obtained from 21 patients with gastric cancer and then examined for their gene expression profiles by the Gene Chip® Human U95Av2 array, which includes 12 000 human genes and EST sequences. A total of 25 genes were upregulated and two genes were downregulated by at least four-fold in the tumour tissue. In a further analysis according to lymph node metastasis, the expressed levels of maspin, as well as carcinoembryonic antigen and nonspecific crossreacting antigen were significantly higher in tumours with lymph node metastasis than in those without it. Maspin expression in 85 gastric cancer patients was further investigated by using immunohistochemistry. Maspin expression was not observed in normal gastric epithelia without intestinal metaplasia. In contrast, maspin was expressed in 74 of 85 tumour tissues. There was a significant correlation between the incidence of maspin-positive tumour staining and lymph node metastasis. These results suggest that maspin has a potential role for tumour metastasis in gastric cancer.
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影响因子：
8
作者：
Heighway, J;Knapp, T;Rickert, P
通讯作者：
Rickert, P
影响因子：
11.2
作者：
Kim, S;Han, JH;Park, C
通讯作者：
Park, C
影响因子：
8
作者：
Xia, WY;Lau, YK;Hung, MC
通讯作者：
Hung, MC
DOI：
10.1016/s0006-291x(02)02136-8
发表时间：
2002-09-13
影响因子：
3.1
作者：
Maass, N;Biallek, M;Nagasaki, K
通讯作者：
Nagasaki, K
影响因子：
2.2
作者：
Son, HJ;Sohn, TS;Rhee, JC
通讯作者：
Rhee, JC