Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS.
Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS.
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同位素标记-RPLC-IM-MS法检测葡萄球菌脂质中的脂肪酸杂环
DOI：
10.1021/jasms.1c00092
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发表时间：
2021-09-01
影响因子：
3.2
通讯作者：
Hines, Kelly M.
中科院分区：
文献类型：
--
作者：
Freeman, Christian;Hynds, Hannah M.;Carpenter, Jana M.;Appala, Keerthi;Bimpeh, Kingsley;Barbarek, Shannon;Gatto, Craig;Wilkinson, Brian J.;Hines, Kelly M.
关键词：
金黄色葡萄球菌膜脂质中高达80%的脂肪酸是支链脂肪酸，而不是直链脂肪酸。支链脂肪酸（BCFA）可以具有偶数或奇数个碳，并且分支位置可以在尾部的倒数第二个碳（iso）或倒数第三个碳（anteiso）。这导致两组具有相同碳数的异构脂肪酸物质，其不能通过质谱法解析。当BCFA和直链脂肪酸（SCFA）的混合物被酯化成二酰化脂质（如S.金黄色膜没有常规的色谱方法能够解析含有SCFA、反异奇、异奇和异偶BCFA混合物的双酰化脂质。这一领域方法开发的一个主要障碍是缺乏含有BCFA异构体的脂质的相关分析标准。S.金黄色葡萄球菌脂质体及其天然高水平的BCFA提供了探索解析含有BCFA和SFCA的二酰化脂质的潜力的机会。利用我们对S.金黄色葡萄球菌，我们已经使用了稳定的同位素标记策略，开发和验证了30分钟C18反相液相色谱法结合行波离子迁移率质谱法，以提供分辨率的基础上，他们包含的BCFA的数量双酰化脂质。
Up to 80% of the fatty acids in Staphylococcus aureus membrane lipids are branched, rather than straight-chain, fatty acids. The branched fatty acids (BCFAs) may have either an even or odd number of carbons, and the branch position may be at the penultimate carbon (iso) or the antepenultimate (anteiso) carbon of the tail. This results in two sets of isomeric fatty acid species with the same number of carbons that cannot be resolved by mass spectrometry. The isomer/isobar challenge is further complicated when the mixture of BCFAs and straight-chain fatty acids (SCFAs) are esterified into diacylated lipids such as the phosphatidylglycerol (PG) species of the S. aureus membrane. No conventional chromatographic method has been able to resolve diacylated lipids containing mixtures of SCFAs, anteiso-odd, iso-odd, and iso-even BCFAs. A major hurdle to method development in this area is the lack of relevant analytical standards for lipids containing BCFA isomers. The diversity of the S. aureus lipidome and its naturally high levels of BCFAs present an opportunity to explore the potential of resolving diacylated lipids containing BCFAs and SFCAs. Using our knowledge of lipid and fatty acid biosynthesis in S. aureus, we have used a stable-isotope-labeling strategy to develop and validate a 30 min C18 reversed-phase liquid chromatography method combined with traveling-wave ion mobility–mass spectrometry to provide resolution of diacylated lipids based on the number of BCFAs that they contain.
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影响因子：
7.4
作者：
Bird, Susan S.;Marur, Vasant R.;Kristal, Bruce S.
通讯作者：
Kristal, Bruce S.
影响因子：
3.1
作者：
David, Frank;Tienpont, Bart;Sandra, Pat
通讯作者：
Sandra, Pat
影响因子：
4.4
作者：
Christinat, Nicolas;Morin-Rivron, Delphine;Masoodi, Mojgan
通讯作者：
Masoodi, Mojgan
影响因子：
5.2
作者：
Hines, Kelly M.;Shen, Tianwei;Xu, Libin
通讯作者：
Xu, Libin
影响因子：
1.8
作者：
Born, Matthias-Erich N.;Prentice, Boone M.
通讯作者：
Prentice, Boone M.