Molecular epidemiology and phylogenetic analysis of Sapporo-like viruses
Molecular epidemiology and phylogenetic analysis of Sapporo-like viruses
复制标题
札幌类病毒的分子流行病学和系统发育分析
作者：
M. Okada;K. Shinozaki;T. Ogawa;I. Kaiho
札幌样病毒（SLV）是人类胃肠炎的病原体。在SLV衣壳蛋白编码区设计特异性引物，对529份胃肠炎患者粪便样品进行检测。逆转录-聚合酶链反应（RT-PCR）检测阳性35例（6.6%）。在35个阳性样本中，25个被归类为基因组SG-I，以札幌病毒为代表，9个被归类为基因组SG-II，以伦敦病毒为代表。有趣的是，一个样本不能被分类到任何基因组，这表明它可能是一个新的SLV基因组的一部分。本研究所用的RT-PCR方法能够广泛检测SLV基因组，为SLV的分子流行病学和系统发育研究提供了有力的工具。
Summary.Sapporo-like viruses (SLV) are a causative agent of gastroenteritis in humans. SLV-specific primers were newly designed in capsid protein-coding region and 529 fecal samples collected from gastroenteritis patients were tested. Thirty-five samples (6.6%) were found to be positive by reverse transcriptase-polymerase chain reaction (RT-PCR). Out of 35 positive samples, 25 were classified into a genogroup SG-I, typified by the Sapporo virus, and 9 were classified as a genogroup SG-II, typified by the London virus. Interestingly, one sample could not be classified into any genogroup, which suggests that it may be part of a new SLV genogroup. The RT-PCR used in this study appeared to be capable of widely detecting SLV genogroups, and it was seen to be powerful enough for molecular epidemiological as well as phylogenetic studies on SLV.
影响因子：
3.7
作者：
JIANG, X;WANG, M;ESTES, MK
通讯作者：
ESTES, MK