Detection and molecular diversity of spike gene of porcine epidemic diarrhea virus in China.
Detection and molecular diversity of spike gene of porcine epidemic diarrhea virus in China.
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中国猪流行性腹泻病毒刺突基因检测及分子多样性
DOI：
10.3390/v5102601
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发表时间：
2013-10-22
期刊：
影响因子：
--
通讯作者：
Feng L
中科院分区：
文献类型：
--
作者：
Chen J;Liu X;Shi D;Shi H;Zhang X;Li C;Chi Y;Feng L
2010年底以来，猪流行性腹泻病毒（PEDV）在中国各地迅速传播，造成新生仔猪发病率高、死亡率高（高达100%）。通过逆转录聚合酶链反应（RT-PCR）证实，29个省（不包括中国西藏和海南）的79.66%（177个中的141个）猪场和72.27%（577个中的417个）样本PEDV呈阳性。对代表性野外菌株的全长S基因进行了测序。 33个野生菌株在核苷酸序列水平上彼此具有93.5%~99.9%的同源性，在氨基酸序列水平上彼此具有92.3%~99.8%的同源性。大多数野生菌株具有核苷酸缺失和插入区域，与中国经典菌株CH/S的同源性较低（93.5%–94.2%），但与新近菌株CHGD-1的同源性较高（97.1%–99.3%）。系统发育分析表明，中国猪群中存在着流行的经典品系和变种。 PEDV在我国猪群中检出率较高。序列分析表明，近期田间菌株的S基因存在异质性，且变异占优势。
Since late 2010, porcine epidemic diarrhea virus (PEDV) has rapidly disseminated all over the China and caused considerable morbidity and high mortality (up to 100%) in neonatal piglets. 79.66% (141 of 177) pig farms in 29 provinces (excluding Tibet and Hainan, China) and 72.27% (417 of 577) samples were positive for PEDV confirmed by reverse transcription-polymerase chain reaction (RT-PCR). The full-length S genes of representative field strains were sequenced. 33 field strains share 93.5%–99.9% homologies with each other at the nucleotide sequence level and 92.3%–99.8% homologies with each other at the amino acids sequence level. Most field strains have nucleotide deletion and insertion regions, and show lower homologies (93.5%–94.2%) with Chinese classical strain CH/S, however higher homologies (97.1%–99.3%) with recent strain CHGD-1. The phylogenetic analysis showed there are classical strains and variants prevailing in pig herd in China. PEDV has a high detection rate in pig herds in China. Sequence analysis indicated the S genes of recent field strains have heterogeneity and the variants are predominant.
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