Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress.
Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress.
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DOI：
10.1038/s41598-017-07852-y
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发表时间：
2017-08-04
影响因子：
4.6
通讯作者：
Li D
中科院分区：
文献类型：
--
作者：
Liu Y;Wang L;Zhang T;Yang X;Li D
脱水蛋白属于第2组莱亚（Late Embryogenesis Abundant）家族蛋白，其在大多数植物中在寒冷和干旱胁迫期间上调。根据Y-、S-和K-片段的数目和顺序，将蛋白质分为五个亚类：YnSKn、YnKn、SKn、Kn和KnS。在这里，玉米（Zea mays L.）KS-type tumor in基因ZmDHN 13被鉴定并随后表征。表达谱分析表明，ZmDHN 13的组成型表达，但其表达也改变了高渗透，低温，氧化胁迫和脱落酸（阿坝）。进一步探讨了这三个保守片段在磷酸化、定位、结合金属离子和生理功能等方面的作用。ZmDHN 13主要定位于细胞核中，这取决于磷酸化状态。进一步的研究表明，当核定位信号（NLS）片段和S片段为核心序列时，ZmDHN 13可以被酪蛋白激酶II（casein kinase II）磷酸化。ZmDHN 13的过表达增强了转基因烟草对氧化胁迫的耐受性，并且这三个保守片段在体内对环境胁迫的响应中表现出协同效应。
Dehydrins belong to the group 2 family LEA (Late Embryogenesis Abundant) proteins, which are up-regulated in most plants during cold and drought stress. According to the number and order of the Y-, S- and K-segments, dehydrins are classified into five subclasses: YnSKn, YnKn, SKn, Kn and KnS. Here, the maize (Zea mays L.) KS-type dehydrin gene, ZmDHN13, was identified and later characterized. Expression profiling demonstrated that ZmDHN13 was constitutively expressed, but its expression was also altered by high osmosis, low temperature, oxidative stress and abscisic acid (ABA). Furthermore, the roles of the three conserved segments in phosphorylation, localization, binding metal ions and physiological functions were explored. ZmDHN13 was mainly localized in the nucleus, depending on phosphorylation status. Additional studies indicated that ZmDHN13 could be phosphorylated by CKII (casein kinase II), when the NLS (nuclear localization signal) segment and the S-segment were core sequences. The overexpression of ZmDHN13 enhanced transgenic tobacco tolerance to oxidative stress, and the three conserved segments exhibited a cooperative effect in response to environmental stresses in vivo.
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影响因子：
5.6
作者：
Hernández-Sánchez IE;Maruri-López I;Ferrando A;Carbonell J;Graether SP;Jiménez-Bremont JF
通讯作者：
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