Airway cell changes in tracheal lavage of sheep after ozone exposure.
Airway cell changes in tracheal lavage of sheep after ozone exposure.
复制标题
臭氧暴露后绵羊气管灌洗液中气道细胞的变化。
DOI：
10.1080/15287398309530366
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
W. Abraham
中科院分区：
文献类型：
--
作者：
M. Sielczak;S. Denas;W. Abraham
我们感兴趣的是臭氧（O3）是否可以刺激肥大细胞迁移到气管腔。为了测试这一点，我们确定了急性O3暴露对气管灌洗回收的细胞类型和相对数量的影响。七只清醒的绵羊被插入细长的鼻气管导管。用10-15-ml等份（共350 ml）0.9%缓冲（pH 7.4）盐水重复灌洗喉和气管套管套囊（15-20 cm）之间的气管，其中含有肥大细胞稳定剂色甘酸二钠（10 μ g/ml）。基线灌洗后1小时，将绵羊分别暴露于空气（对照）或0.5 ppm O3中2小时。24小时后重复灌洗。通过盐水/Ficoll Paque梯度离心从灌洗液中回收细胞。从该材料的一部分，我们估计了总细胞和总活细胞（台盼蓝）。将剩余的材料以400 X g重离心5分钟，并由细胞沉淀制备细胞学载玻片。载玻片用多色染料和瑞-姬染色，并通过光学显微镜进行分析。根据500个细胞/玻片的总计数确定上皮细胞、巨噬细胞、淋巴细胞和肥大细胞的百分比。计算空气和O3暴露前后细胞百分比的差异，并对这些差异进行比较。暴露于O3导致肥大细胞和淋巴细胞的数量增加时，观察到的变化与空气相比。O3暴露后管腔肥大细胞和淋巴细胞数量的增加可能是炎症反应增强的信号，这些变化可能有助于O3诱导的非特异性气道高反应性增加和对IgE介导的过敏性气道反应的易感性。
We were interested in whether ozone (O3) could stimulate the migration of mast cells into the tracheal lumen. To test this we determined the effect of an acute O3 exposure on the types and relative numbers of cells recovered by tracheal lavage. Seven conscious sheep were intubated with an elongated nasotracheal tube. The trachea between the larynx and the cuff of the tracheal tube (15-20 cm) was lavaged repeatedly with 10-15-ml aliquots (total 350 ml) of 0.9% buffered (pH 7.4 saline, which contained the mast cell-stabilizer disodium cromoglycate (10 micrograms/ml). One hour after a baseline lavage, the sheep were exposed on separate occasions to either air (control) or 0.5 ppm O3 for 2 h. Lavages were repeated 24 h later. Cells were recovered from the lavage effluent by centrifugation across a saline/Ficoll Paque gradient. From part of this material we estimated total cells and total viable cells (with Trypan blue). The rest of the material was recentrifuged at 400 X g for 5 min, and cytological slides were made from the cell pellet. Slides were stained with Polichrome and Wright-Giemsa, and were analyzed by light microscopy. The percentages of epithelial cells, macrophages, lymphocytes, and mast cells were determined from a total count of 500 cells/slide. Differences in cell percentages between pre- and postexposure were calculated both for air and O3 exposures, and these differences were compared. Exposure to O3 resulted in an increased number of mast cells and lymphocytes when compared to the changes observed with air. It seems likely that the increase in number of luminal mast cells and lymphocytes following O3 exposure signals an enhanced inflammatory response and that these changes could contribute to O3-induced increased nonspecific airway hyperresponsiveness and susceptibility to allergic IgE-mediated airway reactions.
支气管激发和气管粘液速度检测气道对 O3 反应的敏感性。
DOI：
10.1152/jappl.1980.48.5.789
发表时间：
1980
期刊：
Journal of applied physiology: respiratory, environmental and exercise physiology
影响因子：
--
作者：
Abraham,WM;Januszkiewicz,AJ;Mingle,M;Welker,M;Wanner,A;Sackner,MA
通讯作者：
Sackner,MA