Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis.
Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis.
复制标题
苏云金芽孢杆菌中直接调节的Sigma（54）因子直接调节的代谢途径的鉴定。
DOI：
10.3389/fmicb.2015.00407
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Song F
中科院分区：
文献类型：
--
作者：
Peng Q;Wang G;Liu G;Zhang J;Song F
Sigma54 （σ54）调节细菌对氮和碳的利用。σ54依赖性启动子高度保守，包含位于转录起始位点上游- 24和- 12位置的短序列。σ54需要被称为细菌增强子结合蛋白（bebp）的调节蛋白来激活基因转录。我们发现，利用一个缺少sigL基因编码σ54的苏云金芽孢杆菌HD73突变体，σ54可以调节其在不同氮源上的生长能力（ΔsigL）。采用2倍变化截断和P < 0.05的错误发现率截断对DNA微阵列数据进行分析，发现与野生型HD73菌株相比，ΔsigL突变体中有255个基因下调，121个基因上调。采用DNA芯片、生物信息学和功能分析等方法对固定相σ54调控子进行了表征；在ΔsigL突变体中鉴定出包含47个基因的16个操纵子，其启动子区域包含保守的- 12/ - 24元件，其转录活性被取消或降低。在Bt HD73基因组中发现8个依赖于σ54的转录bebp，它们调控9个依赖于σ54的启动子。在苏云金芽孢杆菌中，σ54在这一过程中激活的代谢途径尚未确定；尽管如此，本文对σ54调控子的分析有助于更好地理解σ因子在细菌中的生理作用。
Sigma54 (σ54) regulates nitrogen and carbon utilization in bacteria. Promoters that are σ54-dependent are highly conserved and contain short sequences located at the −24 and −12 positions upstream of the transcription initiation site. σ54 requires regulatory proteins known as bacterial enhancer-binding proteins (bEBPs) to activate gene transcription. We show that σ54 regulates the capacity to grow on various nitrogen sources using a Bacillus thuringiensis HD73 mutant lacking the sigL gene encoding σ54 (ΔsigL). A 2-fold-change cutoff and a false discovery rate cutoff of P < 0.05 were used to analyze the DNA microarray data, which revealed 255 genes that were downregulated and 121 that were upregulated in the ΔsigL mutant relative to the wild-type HD73 strain. The σ54 regulon (stationary phase) was characterized by DNA microarray, bioinformatics, and functional assay; 16 operons containing 47 genes were identified whose promoter regions contain the conserved −12/−24 element and whose transcriptional activities were abolished or reduced in the ΔsigL mutant. Eight σ54-dependent transcriptional bEBPs were found in the Bt HD73 genome, and they regulated nine σ54-dependent promoters. The metabolic pathways activated by σ54 in this process have yet to be identified in Bacillus thuringiensis; nonetheless, the present analysis of the σ54 regulon provides a better understanding of the physiological roles of σ factors in bacteria.
登录
查看更多内容
影响因子：
3.6
作者：
Heidrich, Nadja;Chinali, Alberto;Brantl, Sabine
通讯作者：
Brantl, Sabine
DOI：
10.1073/pnas.88.20.9092
发表时间：
1991-10-01
影响因子：
11.1
作者：
DEBARBOUILLE, M;MARTINVERSTRAETE, I;RAPOPORT, G
通讯作者：
RAPOPORT, G
影响因子：
3.2
作者：
Belitsky, BR;Sonenshein, AL
通讯作者：
Sonenshein, AL
影响因子：
3.2
作者：
HOPPER, S;BOCK, A
通讯作者：
BOCK, A
影响因子：
5.1
作者：
Cases, I;Ussery, DW;de Lorenzo, V
通讯作者：
de Lorenzo, V