Coupling enrichment methods with proteomics for understanding and treating disease.
Coupling enrichment methods with proteomics for understanding and treating disease.
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DOI：
10.1002/prca.201400097
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发表时间：
2015-02
影响因子：
2
通讯作者：
Betenbaugh, Michael J.
中科院分区：
文献类型：
--
作者：
Kumar, Amit;Baycin-Hizal, Deniz;Shiloach, Joseph;Bowen, Michael A.;Betenbaugh, Michael J.
由于蛋白质组学分析方法和生物信息学能力的最新进展，使用这些能力开发治疗人类疾病的药物的趋势日益增长，包括靶标和药物评价，了解药物作用机制和生物标志物发现。目前，许多主要生物体的基因序列是可用的，这极大地有助于表征模型动物系统和人类中的蛋白质组。通过蛋白质组学，可以表征不同疾病状态的总体概况（例如类型和相对水平的变化以及PTM的变化，如糖基化或磷酸化）。虽然细胞内蛋白质组学可以提供细胞和组织生理学的广泛概述，但难以量化对于理解疾病状态和治疗进展可能很重要的低丰度蛋白质。由于这个原因，有越来越多的兴趣耦合比较蛋白质组学方法与亚细胞分离和富集技术的膜，核，磷酸化蛋白质组，糖蛋白质组以及低丰度血清蛋白。在这篇综述中，我们将提供利用不同的蛋白质组学耦合富集技术的例子，帮助目标和生物标志物的发现，了解药物靶向机制，和mAb的发现。总之，这些改进将有助于更好地了解各种疾病的病理生理学，包括癌症，自身免疫，炎症，心血管疾病和神经系统疾病，以及设计和开发更好的药物来治疗这些疾病。
Owing to recent advances in proteomics analytical methods and bioinformatics capabilities there is a growing trend toward using these capabilities for the development of drugs to treat human disease, including target and drug evaluation, understanding mechanisms of drug action, and biomarker discovery. Currently, the genetic sequences of many major organisms are available, which have helped greatly in characterizing proteomes in model animal systems and humans. Through proteomics, global profiles of different disease states can be characterized (e.g. changes in types and relative levels as well as changes in PTMs such as glycosylation or phosphorylation). Although intracellular proteomics can provide a broad overview of physiology of cells and tissues, it has been difficult to quantify the low abundance proteins which can be important for understanding the diseased states and treatment progression. For this reason, there is increasing interest in coupling comparative proteomics methods with subcellular fractionation and enrichment techniques for membranes, nucleus, phosphoproteome, glycoproteome as well as low abundance serum proteins. In this review, we will provide examples of where the utilization of different proteomics-coupled enrichment techniques has aided target and biomarker discovery, understanding the drug targeting mechanism, and mAb discovery. Taken together, these improvements will help to provide a better understanding of the pathophysiology of various diseases including cancer, autoimmunity, inflammation, cardiovascular disease, and neurological conditions, and in the design and development of better medicines for treating these afflictions.
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影响因子：
3.4
作者：
Chen, Jing;Xi, Jiefeng;Tian, Yuan;Bova, George Steven;Zhang, Hui
通讯作者：
Zhang, Hui
影响因子：
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2
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Nattkemper TW
影响因子：
16
作者：
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通讯作者：
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影响因子：
46.9
作者：
Cheung, Wan Cheung;Beausoleil, Sean A.;Polakiewicz, Roberto D.
通讯作者：
Polakiewicz, Roberto D.