Enrichment of plasma membrane proteins using nanoparticle pellicles: comparison between silica and higher density nanoparticles.
Enrichment of plasma membrane proteins using nanoparticle pellicles: comparison between silica and higher density nanoparticles.
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DOI：
10.1021/pr301107x
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发表时间：
2013-03-01
影响因子：
4.4
通讯作者：
Fenselau C
中科院分区：
文献类型：
--
作者：
Choksawangkarn W;Kim SK;Cannon JR;Edwards NJ;Lee SB;Fenselau C
由于质膜蛋白丰度低、疏水性、频繁羧化和动态群体，质膜蛋白的蛋白质组学和其他表征变得困难。我们和其他人提出，LC-MS/MS 分析中的代表性不足可以通过使用阳离子纳米颗粒薄膜富集质膜及其蛋白质来部分补偿。纳米粒子增加了质膜片的密度，从而增强了通过离心与其他裂解的细胞成分的分离。在此，我们测试了这样的假设：使用密度增加的纳米颗粒可以增强质膜蛋白的富集，以进行蛋白质组分析。多发性骨髓瘤细胞在悬浮液中生长并涂有三种不同密度的三种不同薄膜，并通过高通量 LC-MS/MS 分析薄膜涂覆和未涂覆的悬浮液。通过鉴定的质膜蛋白的总数和光谱计数来评估富集度。
Proteomic and other characterization of plasma membrane proteins is made difficult by their low abundance, hydrophobicity, frequent carboxylation and dynamic population. We and others have proposed that underrepresentation in LC-MS/MS analysis can be partially compensated by enriching the plasma membrane and its proteins using cationic nanoparticle pellicles. The nanoparticles increase the density of plasma membrane sheets and thus enhance separation by centrifugation from other lysed cellular components. Herein we test the hypothesis that the use of nanoparticles with increased densities can provide enhanced enrichment of plasma membrane proteins for proteomic analysis. Multiple myeloma cells were grown and coated in suspension with three different pellicles of three different densities and both pellicle coated and uncoated suspensions analyzed by high-throughput LC-MS/MS. Enrichment was evaluated by the total number and the spectral counts of identified plasma membrane proteins.
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影响因子：
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作者：
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