Human embryonic stem cells derived by somatic cell nuclear transfer.
Human embryonic stem cells derived by somatic cell nuclear transfer.
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DOI：
10.1016/j.cell.2013.05.006
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发表时间：
2013-06-06
期刊：
影响因子：
64.5
通讯作者：
Mitalipov S
中科院分区：
文献类型：
--
作者：
Tachibana M;Amato P;Sparman M;Gutierrez NM;Tippner-Hedges R;Ma H;Kang E;Fulati A;Lee HS;Sritanaudomchai H;Masterson K;Larson J;Eaton D;Sadler-Fredd K;Battaglia D;Lee D;Wu D;Jensen J;Patton P;Gokhale S;Stouffer RL;Wolf D;Mitalipov S
通过体细胞核移植（SCNT）将体细胞重编程为多能胚胎干细胞（ESC）已被设想为用于产生患者匹配的核移植（NT）-ESC的方法，用于疾病机制的研究和用于开发特异性疗法。过去尝试产生人类NT-ESCs失败继发于SCNT胚胎的早期胚胎停滞。在这里，我们确定了人类卵母细胞减数分裂的过早退出和次优激活是导致这些结果的关键因素。旨在规避这些限制的优化SCNT方法允许衍生人NT-ESC。当应用于优质的人卵母细胞时，NT-ESC系来自少至两个卵母细胞。NT-ESCs显示正常的二倍体核型，并完全从亲本体细胞遗传其核基因组。人NT-ESCs的基因表达和分化谱与胚胎来源的ESCs相似，表明体细胞有效重编程为多能状态。
Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state.
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影响因子：
5.2
作者：
Sparman, Michelle;Dighe, Vikas;Sritanaudomchai, Hathaitip;Ma, Hong;Ramsey, Cathy;Pedersen, Darlene;Clepper, Lisa;Nighot, Prashant;Wolf, Don;Hennebold, Jon;Mitalipov, Shoukhrat
通讯作者：
Mitalipov, Shoukhrat
DOI：
10.1089/clo.2008.0038
发表时间：
2008-12-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Nakajima, Noriaki;Inomata, Tomo;Kashiwazaki, Naomi
通讯作者：
Kashiwazaki, Naomi
影响因子：
6.1
作者：
Mitalipov, S. M.;Zhou, Q.;Wolf, D. P.
通讯作者：
Wolf, D. P.
影响因子：
16.6
作者：
Egli, Dieter;Chen, Alice E.;Eggan, Kevin
通讯作者：
Eggan, Kevin
影响因子：
6.1
作者：
PELLICER, A;RUIZ, A;BONILLAMUSOLES, F
通讯作者：
BONILLAMUSOLES, F