Epigenetic reprogramming by somatic cell nuclear transfer in primates.
Epigenetic reprogramming by somatic cell nuclear transfer in primates.
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DOI：
10.1002/stem.60
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发表时间：
2009-06
期刊：
影响因子：
5.2
通讯作者：
Mitalipov, Shoukhrat
中科院分区：
文献类型：
--
作者：
Sparman, Michelle;Dighe, Vikas;Sritanaudomchai, Hathaitip;Ma, Hong;Ramsey, Cathy;Pedersen, Darlene;Clepper, Lisa;Nighot, Prashant;Wolf, Don;Hennebold, Jon;Mitalipov, Shoukhrat
我们最近证明，成年灵长类动物的体细胞可以通过体细胞核移植重新编程为多能状态。然而，来自一只猴子的供体细胞的低效率需要大量的卵母细胞。在这里，我们证明了不同核供体细胞的囊胚发育和胚胎干（ES）细胞衍生率高出近三倍。使用成年雌性恒河猴皮肤成纤维细胞作为核供体和从一只雌性动物中取出的卵母细胞，在单次控制性卵巢刺激后分离出两种ES细胞系。除了涉及体外和体内分化成各种体细胞类型的常规多能性测试之外，源自重编程体细胞的灵长类动物ES细胞也能够有助于表达生殖细胞标记物的细胞。此外，印迹基因表达，甲基化，端粒长度和X-失活分析与卵母细胞特异性因子对体细胞的精确和广泛的表观遗传重编程一致。
We recently demonstrated that somatic cells from adult primates could be reprogrammed into a pluripotent state by somatic cell nuclear transfer. However, the low efficiency with donor cells from one monkey necessitated the need for large oocyte numbers. Here, we demonstrate nearly threefold higher blastocyst development and embryonic stem (ES) cell derivation rates with different nuclear donor cells. Two ES cell lines were isolated using adult female rhesus macaque skin fibroblasts as nuclear donors and oocytes retrieved from one female, following a single controlled ovarian stimulation. In addition to routine pluripotency tests involving in vitro and in vivo differentiation into various somatic cell types, primate ES cells derived from reprogrammed somatic cells were also capable of contributing to cells expressing markers of germ cells. Moreover, imprinted gene expression, methylation, telomere length, and X-inactivation analyses were consistent with accurate and extensive epigenetic reprogramming of somatic cells by oocyte-specific factors.
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影响因子：
5.2
作者：
Hoffman, LM;Hall, L;Carpenter, MK
通讯作者：
Carpenter, MK
影响因子：
6.1
作者：
Mitalipov, S. M.;Zhou, Q.;Wolf, D. P.
通讯作者：
Wolf, D. P.
影响因子：
5.2
作者：
Mitalipov, Shoukhrat;Clepper, Lisa;Wolf, Don
通讯作者：
Wolf, Don
影响因子：
2.5
作者：
Oback, B.;Wells, D. N.
通讯作者：
Wells, D. N.
影响因子：
30.8
作者：
Rideout, WM;Wakayama, T;Jaenisch, R
通讯作者：
Jaenisch, R