Comparative tRNA sequencing and RNA mass spectrometry for surveying tRNA modifications.
Comparative tRNA sequencing and RNA mass spectrometry for surveying tRNA modifications.
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DOI：
10.1038/s41589-020-0558-1
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发表时间：
2020-09
影响因子：
14.8
通讯作者：
Waldor MK
中科院分区：
文献类型：
--
作者：
Kimura S;Dedon PC;Waldor MK
构成tRNA的核苷的化学修饰是多样的。然而，结构，位置和程度的修改已被系统地绘制在极少数生物。在这里，我们描述了一种方法，其中通过高通量tRNA测序（tRNA-seq）数据中的逆转录衍生签名快速预测修饰位点，并通过RNA质谱法鉴定tRNA修饰。比较tRNA-seq能够预测E. coli中进行了比较，并揭示了各种环境条件对霍乱弧菌tRNA修饰的影响。通过RNA质谱分析，我们发现其中两个霍乱弧菌特异性逆转录特征反映了新修饰（乙酰化acp 3U（acacp 3U））的存在，而其他结果来自C-到-cRNA编辑，这是一个以前没有描述过的过程。这些发现证明了这种方法用于快速监测tRNA修饰谱和tRNA修饰的环境控制的实用性。
Chemical modifications of the nucleosides that comprise tRNAs are diverse. However, the structure, location, and extent of modifications have been systematically charted in very few organisms. Here, we describe an approach in which rapid prediction of modified sites through reverse transcription-derived signatures in high-throughput tRNA-sequencing (tRNA-seq) data is coupled with identification of tRNA modifications through RNA mass spectrometry. Comparative tRNA-seq enabled prediction of several V. cholerae modifications that are absent from E. coli and also revealed the effects of various environmental conditions on V. cholerae tRNA modification. Through RNA mass spectrometric analyses, we showed that two of the V. cholerae-specific reverse transcription signatures reflected the presence of a new modification (acetylated acp3U (acacp3U)), while the other results from C-to-Ψ RNA editing, a process not described before. These findings demonstrate the utility of this approach for rapid surveillance of tRNA modification profiles and environmental control of tRNA modification.
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影响因子：
14.9
作者：
Chan PP;Lowe TM
通讯作者：
Lowe TM
影响因子：
64.5
作者：
Gingold, Hila;Tehler, Disa;Pilpel, Yitzhak
通讯作者：
Pilpel, Yitzhak
DOI：
10.1073/pnas.0803281105
发表时间：
2008-06-24
影响因子：
11.1
作者：
Cameron, D. Ewen;Urbach, Jonathan M.;Mekalanos, John J.
通讯作者：
Mekalanos, John J.
影响因子：
14.9
作者：
Jühling F;Mörl M;Hartmann RK;Sprinzl M;Stadler PF;Pütz J
通讯作者：
Pütz J
影响因子：
3.1
作者：
DONNENBERG, MS;KAPER, JB
通讯作者：
KAPER, JB