GLOBAL AND TARGETED PROFILING OF GTP-BINDING PROTEINS IN BIOLOGICAL SAMPLES BY MASS SPECTROMETRY.
GLOBAL AND TARGETED PROFILING OF GTP-BINDING PROTEINS IN BIOLOGICAL SAMPLES BY MASS SPECTROMETRY.
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DOI：
10.1002/mas.21637
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发表时间：
2021-05
影响因子：
6.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Huang M;Wang Y
GTP 结合蛋白是参与多种生物过程的最重要的酶家族之一。然而，由于核苷酸结合蛋白家族的巨大多样性，使用传统方法对GTP结合蛋白的表达水平、结构、活性和调控机制进行全面分析仍然具有挑战性。质谱 (MS) 仪器和数据采集方法的许多进步，加上样品制备中的各种富集方法，使 MS 成为综合表征各种 GTP 结合蛋白的活性和表达水平的强大工具。我们在此回顾了基于 MS 的技术应用的最新进展，以及通用和广泛使用的亲和富集方法，用于全蛋白质组和靶向捕获、鉴定和定量 GTP 结合蛋白。讨论了分析 GTP 结合蛋白的表达水平、活性、翻译后修饰和相互作用组的各种策略的工作原理、优点和局限性。可以预见，基于 MS 的蛋白质组学的未来应用将有助于更好地了解 GTP 结合蛋白在不同生物过程和人类疾病中的作用。
GTP-binding proteins are among the most important enzyme families that are involved in a plethora of biological processes. However, owing to the enormous diversity of the nucleotide-binding protein family, comprehensive analyses of the expression level, structure, activity, and regulatory mechanisms of GTP-binding proteins remain challenging with the use of conventional approaches. The many advances in mass spectrometry (MS) instrumentation and data acquisition methods, together with a variety of enrichment approaches in sample preparation, render MS a powerful tool for the comprehensive characterizations of the activities and expression levels of various GTP-binding proteins. We review herein the recent developments in the application of MS-based techniques, together with general and widely used affinity enrichment approaches, for the proteome-wide and targeted capture, identification and quantification of GTP-binding proteins. The working principles, advantages, and limitations of various strategies for profiling the expression level, activity, post-translational modifications, and interactome of GTP-binding proteins are discussed. It can be envisaged that future applications of MS-based proteomics will lead to a better understanding about the roles of GTP-binding proteins in different biological processes and human diseases.
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影响因子：
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作者：
Charron G;Tsou LK;Maguire W;Yount JS;Hang HC
通讯作者：
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DOI：
10.1016/j.bbrc.2014.02.041
发表时间：
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