Top Down proteomics: facts and perspectives.
Top Down proteomics: facts and perspectives.
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DOI：
10.1016/j.bbrc.2014.02.041
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发表时间：
2014-03-21
影响因子：
3.1
通讯作者：
Kelleher, Neil L.
中科院分区：
文献类型：
--
作者：
Catherman, Adam D.;Skinner, Owen S.;Kelleher, Neil L.
“自顶向下”方法在基于质谱的蛋白质组学领域的兴起，为蛋白质的表征和鉴定带来了一个充满希望和挑战的新时代。将完整的蛋白质注射到质谱仪中可以更好地表征翻译后修饰，并避免了与基于肽的蛋白质组学相关的几个严重的“推断”问题。然而，对内源性或其他生物相关样品成功实施自顶向下方法通常需要在质谱分析之前使用一种或多种分离形式。最近在仪器方面的进步已经与使用光子和电子的新离子碎片相结合，这使得在几年前根本无法处理的情况下，可以更好地（通常是完整的）表征蛋白质。最后，使用天然电喷雾质谱法在鉴定和表征100 kDa至1 MDa范围内的全蛋白复合物方面显示出巨大的前景，在未来几年内，对内源性蛋白质组装体进行完整的成分分析是一个可行的目标。
The rise of the “Top Down” method in the field of mass spectrometry-based proteomics has ushered in a new age of promise and challenge for the characterization and identification of proteins. Injecting intact proteins into the mass spectrometer allows for better characterization of post-translational modifications and avoids several of the serious “inference” problems associated with peptide-based proteomics. However, successful implementation of a Top Down approach to endogenous or other biologically relevant samples often requires the use of one or more forms of separation prior to mass spectrometric analysis, which have only begun to mature for whole protein MS. Recent advances in instrumentation have been used in conjunction with new ion fragmentation using photons and electrons that allow for better (and often complete) protein characterization on cases simply not tractable even just a few years ago. Finally, the use of native electrospray mass spectrometry has shown great promise for the identification and characterization of whole protein complexes in the 100 kDa to 1 MDa regime, with prospects for complete compositional analysis for endogenous protein assemblies a viable goal over the coming few years.
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影响因子：
64.5
作者：
Cox, Juergen;Mann, Matthias
通讯作者：
Mann, Matthias
DOI：
10.1016/s0021-9673(00)94785-x
发表时间：
1988-06-29
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ALPERT, AJ;ANDREWS, PC
通讯作者：
ANDREWS, PC
DOI：
10.1073/pnas.0607719103
发表时间：
2006-10-31
影响因子：
11.1
作者：
Carroll, Joe;Fearnley, Ian M.;Walker, John E.
通讯作者：
Walker, John E.
影响因子：
2
作者：
Chong, BE;Yan, F;Miller, FR
通讯作者：
Miller, FR
影响因子：
2.8
作者：
COMISARO.MB;MARSHALL, AG
通讯作者：
MARSHALL, AG