Study on RNAi-based herbicide for Mikania micrantha.
Study on RNAi-based herbicide for Mikania micrantha.
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基于RNAi的薇甘菊除草剂的研究
DOI：
10.1016/j.synbio.2021.11.005
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发表时间：
2021-12
影响因子：
4.8
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Mai J;Liao L;Ling R;Guo X;Lin J;Mo B;Chen W;Yu Y
来自南美洲的入侵植物薇甘菊(M.micrantha)对生态系统的稳定性和生物多样性构成重大威胁。然而，目前还缺乏一种经济有效的防治薇甘菊的方法。RNA干扰(RNAi)已被广泛研究和应用于农业性状改良。喷雾诱导基因沉默(SIGS)可以在不对植物基因组引入可遗传修饰的情况下产生RNAi沉默效应，正在成为一种新的非转化植物保护策略。在本研究中，通过对薇甘菊转录组的高通量测序和序列特异性的生物信息学分析，选择编码叶绿素a/b结合蛋白的基因作为RNAi的靶标。设计并合成了3种类型的RNAi分子，即双链RNA、RNAi纳米微球和短发夹状RNA(ShRNA)及其相应的短干扰RNA序列，用于构建Sigs载体，每个RNAi分子被转录和提取后喷洒在薇甘菊叶片上。而水处理的对照叶片保持绿色，而RNAi分子处理的叶片变黄并最终枯萎。实时定量聚合酶链式反应显示，与对照组相比，RNAi处理组目标基因的表达水平显著降低，表明三种类型的RNAi除草剂都有效地沉默了对薇甘菊生长至关重要的内源基因。我们还发现shRNA比其他两个分子具有更好的沉默效率。综上所述，我们的研究成功地设计了三种基于RNAi的除草剂，它们特异性地沉默了内源靶基因，并控制了薇甘菊的生长。此外，我们还发现了一个编码叶绿素a/b结合蛋白的基因家族，该基因家族对薇甘菊的生长发育具有重要作用，可以作为控制薇甘菊传播的潜在靶标。
The invasive plant Mikania micrantha Kunth (M. micrantha) from South America poses a significant threat to the stability and biodiversity of ecosystems. However, an effective and economical method to control M. micrantha is still lacking. RNA interference (RNAi) has been widely studied and applied in agriculture for trait improvement. Spray-induced gene silencing (SIGS) can produce RNAi silencing effects without introducing heritable modifications to the plant genome and is becoming a novel nontransformation strategy for plant protection. In this study, the genes encoding chlorophyll a/b-binding proteins were selected as targets of RNAi, based on high-throughput sequencing of M. micrantha transcriptome and bioinformatic analyses of sequence specificity. Three types of RNAi molecules, double-stranded RNA, RNAi nanomicrosphere, and short hairpin RNA (shRNA), with their corresponding short interfering RNA sequences were designed and synthesized for SIGS vector construction, from which each RNAi molecule was transcribed and extracted to be sprayed on M. micrantha leaves. Whereas water-treated control leaves remained green, leaves treated with RNAi molecules turned yellow and eventually wilted. Quantitative real-time PCR showed that the expression levels of target genes were significantly reduced in the RNAi-treated groups compared with those of the control, suggesting that all three types of RNAi herbicides effectively silenced the endogenous target genes, which are essential for the growth of M. micrantha. We also found that shRNA showed better silencing efficiency than the other two molecules. Taken together, our study successfully designed three types of RNAi-based herbicides that specifically silenced endogenous target genes and controlled the growth of M. micrantha. Moreover, we identified a gene family encoding chlorophyll a/b-binding proteins that is important for the growth and development of M. micrantha and could serve as potential targets for controlling the spread of M. micrantha.
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DOI：
10.1016/j.jiec.2018.10.013
发表时间：
2019-02-25
影响因子：
6.1
作者：
Jeon, Hyunsu;Han, Sangwoo;Lee, Jong Bum
通讯作者：
Lee, Jong Bum
影响因子：
16.6
作者：
Ishibashi, Kazuhiro;Saruta, Masayasu;Kaga, Akito
通讯作者：
Kaga, Akito
DOI：
10.1080/09670878209370717
发表时间：
1982-01-01
期刊：
TROPICAL PEST MANAGEMENT
影响因子：
--
作者：
COCK, MJW
通讯作者：
COCK, MJW
影响因子：
16.6
作者：
Liu, Bo;Yan, Jian;Wan, Fanghao
通讯作者：
Wan, Fanghao
影响因子：
3.4
作者：
Mattei, Bruno;Lira, Rafael B.;Riske, Karin A.
通讯作者：
Riske, Karin A.