Detection of dental fomites using topical fluorescein.
Detection of dental fomites using topical fluorescein.
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DOI：
10.1038/s41415-022-4403-7
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发表时间：
2022-07-05
影响因子：
2.6
通讯作者：
Louca, Chris
中科院分区：
文献类型：
--
作者：
Newsom, Richard;Pattison, Chris;Amara, Adam;Louca, Chris
背景在新冠肺炎大流行期间，牙科设施的彻底消毒至关重要。患者、临床医生、学生和护士都可以被含有新冠肺炎的气雾剂和牙滴感染。然而，水滴是透明的，而且往往是微观的，因此在临床实践中很难检测到。方法为了更好地了解牙滴的传播情况，我们用荧光素对牙科冲洗剂进行了染色，并在牙科假人上进行了一系列的操作。然后，我们观察了在有和没有紫外线(UV)光的情况下，牙科椅子周围散布的水滴和甲藻。结果在无紫外光照射的情况下，观察到液体扩散很少或没有。然而，使用紫外光，我们检测到牙医、椅子和手机上的荧光素，以及地板和仪器托盘上的飞溅。这对工作人员具有教育价值，他们被提醒水滴已经扩散到了什么程度。结论荧光素有助于检测液滴扩散，帮助临床工作人员发现需要深入清洁的高危区域。由于临床级荧光素价格便宜且可广泛获得，这项技术可能对牙科实践有用，以培训工作人员彻底净化临床环境。牙滴扩散已经有了很好的记录；然而，在正常实践中，它是无法检测到的。使用荧光素可以检测到甲壳素和液滴扩散。彻底清洁表面可减少霍乱菌传播，降低新冠肺炎对患者和所有牙科团队成员的风险。
Background Thorough disinfection of dental facilities is of paramount importance during the COVID-19 pandemic. Patients, clinicians, students and nurses can all be infected by aerosols and dental droplets bearing COVID-19. However, droplets are transparent and often microscopic, so are difficult to detect in clinical practice. Methods To better understand the spread of dental droplets, we stained the dental irrigant with fluorescein and performed a series of procedures on a dental manikin. We then viewed droplets and fomite spread around the dental chair, with and without an ultraviolet (UV) light. Results Observations without the UV light showed minimal or no fluid spread. However, using UV light, we detected fluorescein on the dentist, chairs and the handpiece, as well as splatter on the floor and on the instrument tray. This was of educational value to the staff, who were reminded how far droplets had spread. Conclusion Fluorescein facilitates the detection of droplet spread and helps clinical staff to see high-risk areas that require in-depth cleaning. As clinical grade fluorescein is cheap and widely available, this technique may be useful for dental practices to train staff in the thorough decontamination of the clinical environment. Dental droplet spread has been well documented; however, in normal practice, it is undetectable. Use of fluorescein enables detection of fomite and droplet spread. Thorough cleaning of surfaces reduces fomite spread and the risk of COVID-19 to patients and all dental team members.
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影响因子：
18.2
作者：
Walker JS;Archer J;Gregson FKA;Michel SES;Bzdek BR;Reid JP
通讯作者：
Reid JP
影响因子：
2.6
作者：
Llandro H;Allison JR;Currie CC;Edwards DC;Bowes C;Durham J;Jakubovics N;Rostami N;Holliday R
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Holliday R
DOI：
10.1308/003588406x98577
发表时间：
2006-05-01
影响因子：
1.4
作者：
Macdonald, Duncan J. M.;Mckillop, Elisabeth C. A.;Gray, Alastair Jr
通讯作者：
Gray, Alastair Jr
影响因子：
5.1
作者：
SCANLON, JW;LEIKKANEN, M
通讯作者：
LEIKKANEN, M
DOI：
10.14219/jada.archive.1994.0093
发表时间：
1994-05-01
影响因子：
3.9
作者：
BENTLEY, CD;BURKHART, NW;CRAWFORD, JJ
通讯作者：
CRAWFORD, JJ