Fluorescent single-stranded DNA-based assay for detecting unchelated Gadolinium(III) ions in aqueous solution.
Fluorescent single-stranded DNA-based assay for detecting unchelated Gadolinium(III) ions in aqueous solution.
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DOI：
10.1007/s00216-016-9503-2
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发表时间：
2016-06
影响因子：
4.3
通讯作者：
Halim M
中科院分区：
文献类型：
--
作者：
Edogun O;Nguyen NH;Halim M
The main concern pertaining to the safety of Gadolinium(III) Based Contrast Agents (GBCAs) is the toxicity caused by the unchelated ion, which may be inadvertently present in the solution due most commonly, to excess unreacted starting material or dissociation of the complexes. Detecting the aqueous free ion during the synthesis and preparation of GBCA solutions is therefore instrumental in ensuring the safety of the agents. This paper reports the development of a sensitive fluorogenic sensor for aqueous unchelated Gadolinium(III) (Gd(III)). Our design utilizes single-stranded oligodeoxynucleotides with a specific sequence of 44 bases as the targeting moiety. The fluorescence-based assay may be run at ambient pH with very small amount of samples in 384-well plates. The sensor is able to detect nanoMolar concentration of Gd(III), and is relatively unresponsive toward a range of biologically relevant ions and the chelated Gd(III). Although some cross-reactivity with other trivalent lanthanide ions, such as Europium(III) and Terbium(III) is observed, these are not commonly found in biological systems and contrast agents. This convenient and rapid method may be useful in ascertaining a high purity of GBCA solutions.
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通讯作者：
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