Human rhinovirus infection causes different DNA methylation changes in nasal epithelial cells from healthy and asthmatic subjects.
Human rhinovirus infection causes different DNA methylation changes in nasal epithelial cells from healthy and asthmatic subjects.
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DOI：
10.1186/1755-8794-7-37
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发表时间：
2014-06-19
影响因子：
2.7
通讯作者：
Avila PC
中科院分区：
文献类型：
--
作者：
McErlean P;Favoreto S Jr;Costa FF;Shen J;Quraishi J;Biyasheva A;Cooper JJ;Scholtens DM;Vanin EF;de Bonaldo MF;Xie H;Soares MB;Avila PC
Mechanisms underlying the development of virus-induced asthma exacerbations remain unclear. To investigate if epigenetic mechanisms could be involved in virus-induced asthma exacerbations, we undertook DNA methylation profiling in asthmatic and healthy nasal epithelial cells (NECs) during Human Rhinovirus (HRV) infection in vitro. Global and loci-specific methylation profiles were determined via Alu element and Infinium Human Methylation 450 K microarray, respectively. Principal components analysis identified the genomic loci influenced the most by disease-status and infection. Real-time PCR and pyrosequencing were used to confirm gene expression and DNA methylation, respectively. HRV infection significantly increased global DNA methylation in cells from asthmatic subjects only (43.6% to 44.1%, p = 0.04). Microarray analysis revealed 389 differentially methylated loci either based on disease status, or caused by virus infection. There were disease-associated DNA methylation patterns that were not affected by HRV infection as well as HRV-induced DNA methylation changes that were unique to each group. A common methylation locus stood out in response to HRV infection in both groups, where the small nucleolar RNA, H/ACA box 12 (SNORA12) is located. Further analysis indicated that a relationship existed between SNORA12 DNA methylation and gene expression in response to HRV infection. We describe for the first time that Human rhinovirus infection causes DNA methylation changes in airway epithelial cells that differ between asthmatic and healthy subjects. These epigenetic differences may possibly explain the mechanism by which respiratory viruses cause asthma exacerbations.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1164/rccm.200907-1071oc
发表时间：
2010-05-01
影响因子：
24.7
作者：
Kicic, Anthony;Hallstrand, Teal S.;Knight, Darryl A.
通讯作者：
Knight, Darryl A.
影响因子：
9.6
作者：
Sood, Akshay;Petersen, Hans;Tesfaigzi, Yohannes
通讯作者：
Tesfaigzi, Yohannes
DOI：
10.1164/rccm.200807-1097oc
发表时间：
2009-04-01
影响因子：
24.7
作者：
Baccarelli, Andrea;Wright, Robert O.;Schwartz, Joel
通讯作者：
Schwartz, Joel
DOI：
10.1513/pats.200907-072dp
发表时间：
2009-12-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Holgate, Stephen T;Roberts, Graham;Davies, Donna E
通讯作者：
Davies, Donna E