Comparative Transcriptome Analyses Reveal Potential Mechanisms of Enhanced Drought Tolerance in Transgenic Salvia Miltiorrhiza Plants Expressing AtDREB1A from Arabidopsis.
Comparative Transcriptome Analyses Reveal Potential Mechanisms of Enhanced Drought Tolerance in Transgenic Salvia Miltiorrhiza Plants Expressing AtDREB1A from Arabidopsis.
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DOI：
10.3390/ijms19030827
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发表时间：
2018-03-12
影响因子：
5.6
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Wei T;Deng K;Wang H;Zhang L;Wang C;Song W;Zhang Y;Chen C
在我们前期的研究中，通过过量表达转录因子AtDREB 1A获得了丹参抗旱转基因植株。为了揭示转基因植物耐旱性提高的分子机制，在本研究中，我们使用RNA测序（RNA-seq）比较了野生型（WT）和AtDREB 1A表达转基因植物的全局转录谱。利用聚类分析，我们确定了3904差异表达基因（DEG）。与野生型相比，干旱处理前pRD 29 A：：AtDREB 1A-31中有423个unigenes表达上调，干旱处理6 d后有936个unigenes表达下调，1580和1313个unigenes表达上调和下调。COG分析表明，“信号转导机制”的类别是高度丰富的，在这些DEG干旱胁迫前后。根据京都基因与基因组百科全书（KEGG）的注释，推测与“核糖体”、“植物激素信号转导”、“光合作用”、“植物-病原体相互作用”、“糖酵解/光合异生”和“碳固定”相关的DEG在AtDREB 1A转基因植物的抗旱性中发挥主要功能。干旱胁迫后，与不同转录因子相关的DEG数量显著增加，尤其是AP 2/ERF、bZIP和MYB蛋白家族。总之，这项研究大大扩展了S。为阐明AtDREB 1A介导的转基因植物耐旱机制提供了有价值的线索。
In our previous study, drought-resistant transgenic plants of Salvia miltiorrhiza were produced via overexpression of the transcription factor AtDREB1A. To unravel the molecular mechanisms underpinning elevated drought tolerance in transgenic plants, in the present study we compared the global transcriptional profiles of wild-type (WT) and AtDREB1A-expressing transgenic plants using RNA-sequencing (RNA-seq). Using cluster analysis, we identified 3904 differentially expressed genes (DEGs). Compared with WT plants, 423 unigenes were up-regulated in pRD29A::AtDREB1A-31 before drought treatment, while 936 were down-regulated and 1580 and 1313 unigenes were up- and down-regulated after six days of drought. COG analysis revealed that the ‘signal transduction mechanisms’ category was highly enriched among these DEGs both before and after drought stress. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, DEGs associated with “ribosome”, “plant hormone signal transduction”, photosynthesis”, “plant-pathogen interaction”, “glycolysis/gluconeogenesis” and “carbon fixation” are hypothesized to perform major functions in drought resistance in AtDREB1A-expressing transgenic plants. Furthermore, the number of DEGs associated with different transcription factors increased significantly after drought stress, especially the AP2/ERF, bZIP and MYB protein families. Taken together, this study substantially expands the transcriptomic information for S. miltiorrhiza and provides valuable clues for elucidating the mechanism of AtDREB1A-mediated drought tolerance in transgenic plants.
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影响因子：
5.6
作者：
Li Z;Hu G;Liu X;Zhou Y;Li Y;Zhang X;Yuan X;Zhang Q;Yang D;Wang T;Zhang Z
通讯作者：
Zhang Z
影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
5.6
作者：
Czemmel S;Höll J;Loyola R;Arce-Johnson P;Alcalde JA;Matus JT;Bogs J
通讯作者：
Bogs J
影响因子：
5.6
作者：
Deb A;Grewal RK;Kundu S
通讯作者：
Kundu S
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影响因子：
13.8
作者：
Gong, Biao;Li, Xiu;Wang, Xiufeng
通讯作者：
Wang, Xiufeng