Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize.
Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize.
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DOI：
10.3389/fpls.2016.01477
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li Z;Hu G;Liu X;Zhou Y;Li Y;Zhang X;Yuan X;Zhang Q;Yang D;Wang T;Zhang Z
玉米起源于热带气候，随着种植范围扩大到世界大部分温带地区，玉米面临着巨大的挑战。在这些地区，霜冻和低温是阻止玉米充分发挥其产量潜力的主要因素。在适合北方的30个优良玉米自交系中，我们鉴定了两个极端但相反的耐冻性水平-高度耐受和高度敏感。在这两个品系的苗期，我们使用RNA-seq测量冷冻处理前后玉米全基因组转录组的变化。总共表达了19，794个基因，其中4550个由于处理（冷冻前或冷冻后）或品系类型（耐受或敏感）而表现出差异表达。在4550个差异表达的基因中，948个由于在冷冻条件下处理而表现出差异表达。基因本体分析发现，这948个基因的结合功能（DNA结合，ATP结合，金属离子结合），蛋白激酶活性，肽酶活性显着富集。基于它们的富集、文献支持和显著水平的差异表达，从这948个基因中选择30个进行定量实时PCR（qRT-PCR）验证。验证证实了我们基于RNA-Seq的发现，耐受性和敏感性品系的平方相关系数分别为80%和50%。该研究为进一步研究玉米早期冰冻反应的分子机制提供了宝贵的资源，并为培育具有上级抗冻性的品种以实现产量潜力提供了有针对性的育种策略。
Originating in a tropical climate, maize has faced great challenges as cultivation has expanded to the majority of the world's temperate zones. In these zones, frost and cold temperatures are major factors that prevent maize from reaching its full yield potential. Among 30 elite maize inbred lines adapted to northern China, we identified two lines of extreme, but opposite, freezing tolerance levels—highly tolerant and highly sensitive. During the seedling stage of these two lines, we used RNA-seq to measure changes in maize whole genome transcriptome before and after freezing treatment. In total, 19,794 genes were expressed, of which 4550 exhibited differential expression due to either treatment (before or after freezing) or line type (tolerant or sensitive). Of the 4550 differently expressed genes, 948 exhibited differential expression due to treatment within line or lines under freezing condition. Analysis of gene ontology found that these 948 genes were significantly enriched for binding functions (DNA binding, ATP binding, and metal ion binding), protein kinase activity, and peptidase activity. Based on their enrichment, literature support, and significant levels of differential expression, 30 of these 948 genes were selected for quantitative real-time PCR (qRT-PCR) validation. The validation confirmed our RNA-Seq-based findings, with squared correlation coefficients of 80% and 50% in the tolerance and sensitive lines, respectively. This study provided valuable resources for further studies to enhance understanding of the molecular mechanisms underlying maize early freezing response and enable targeted breeding strategies for developing varieties with superior frost resistance to achieve yield potential.
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