Cbp3-Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly.
Cbp3-Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly.
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DOI：
10.1083/jcb.201103132
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发表时间：
2011-06-13
期刊：
影响因子：
--
通讯作者：
Ott M
中科院分区：
文献类型：
--
作者：
Gruschke S;Kehrein K;Römpler K;Gröne K;Israel L;Imhof A;Herrmann JM;Ott M
呼吸链组分细胞色素B的生物合成所需的一种特殊复合物与酵母线粒体核糖体的隧道出口结合，以协调蛋白质的合成和组装。线粒体包含其自身的遗传系统以表达少量疏水多肽，包括细胞色素B，呼吸链的bc 1复合物的必需亚基。在本文中，我们表明，在酵母中，Cbp 3，一个bc 1复合装配因子，和Cbp 6，细胞色素B翻译的调节剂，形成一个复合物，与线粒体核糖体的多肽隧道出口，并表现出两个重要的功能，在细胞色素B的生物合成。一方面，Cbp 3和Cbp 6与线粒体核糖体的相互作用是细胞色素B转录本高效翻译所必需的。另一方面，Cbp 3-Cbp 6复合物直接与新合成的细胞色素B在装配中间体中相互作用，所述装配中间体不与核糖体结合并且含有装配因子Cbp 4。我们的研究结果表明，细胞色素B的合成优先发生在那些核糖体上的Cbp 3-Cbp 6复合物绑定到他们的隧道出口，一种安排，可以确保细胞色素B的合成和组装的紧密协调。
A complex specifically required for the biogenesis of the respiratory chain component cytochrome b binds to the tunnel exit of yeast mitochondrial ribosomes to coordinate protein synthesis and assembly. Mitochondria contain their own genetic system to express a small number of hydrophobic polypeptides, including cytochrome b, an essential subunit of the bc1 complex of the respiratory chain. In this paper, we show in yeast that Cbp3, a bc1 complex assembly factor, and Cbp6, a regulator of cytochrome b translation, form a complex that associates with the polypeptide tunnel exit of mitochondrial ribosomes and that exhibits two important functions in the biogenesis of cytochrome b. On the one hand, the interaction of Cbp3 and Cbp6 with mitochondrial ribosomes is necessary for efficient translation of cytochrome b transcript. On the other hand, the Cbp3–Cbp6 complex interacts directly with newly synthesized cytochrome b in an assembly intermediate that is not ribosome bound and that contains the assembly factor Cbp4. Our results suggest that synthesis of cytochrome b occurs preferentially on those ribosomes that have the Cbp3–Cbp6 complex bound to their tunnel exit, an arrangement that may ensure tight coordination of cytochrome b synthesis and assembly.
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影响因子：
11.4
作者：
Barrientos, A;Zambrano, A;Tzagoloff, A
通讯作者：
Tzagoloff, A
影响因子：
64.8
作者：
Bingel-Erlenmeyer, Rouven;Kohler, Rebecca;Ban, Nenad
通讯作者：
Ban, Nenad
DOI：
10.1073/pnas.85.8.2677
发表时间：
1988-04-01
影响因子：
11.1
作者：
COSTANZO, MC;FOX, TD
通讯作者：
FOX, TD
DOI：
10.1073/pnas.78.3.1778
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DHAWALE, S;HANSON, DK;MAHLER, HR
通讯作者：
MAHLER, HR
影响因子：
5.3
作者：
Green-Willms, NS;Fox, TD;Costanzo, MC
通讯作者：
Costanzo, MC