The rubber tree genome shows expansion of gene family associated with rubber biosynthesis.
The rubber tree genome shows expansion of gene family associated with rubber biosynthesis.
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DOI：
10.1038/srep28594
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发表时间：
2016-06-24
影响因子：
4.6
通讯作者：
Matsui M
中科院分区：
文献类型：
--
作者：
Lau NS;Makita Y;Kawashima M;Taylor TD;Kondo S;Othman AS;Shu-Chien AC;Matsui M
巴西橡胶树精氨酸是大戟科植物中的一员，是唯一一种用于商业化生产高品质天然橡胶的天然资源。天然胶乳的性能在许多工业应用中几乎是合成胶乳不可替代的。橡胶高产性状生物合成的分子机制仍然缺乏认识。在这里，我们报告了广泛种植的H。brasiliensis clone，RRIM 600.基因组是基于Illumina和PacBio序列数据的~155倍组合覆盖率组装的，总长度为1.55 Gb，其中72.5%包含重复DNA序列。总共预测了84，440个高置信度的蛋白质编码基因。比较基因组分析显示，H. brasiliensis和其他大戟科基因组。我们的数据表明H. brasiliensis产生高水平胶乳的能力可归因于其基因组中橡胶生物合成相关基因的扩增以及这些基因在胶乳中的高表达。利用帽分析基因表达数据，我们说明了橡胶生物合成相关基因的组织特异性转录谱，揭示了转录调控的替代手段。本研究加深了对H.巴西橡胶树生物学的研究，为橡胶树的农艺学改良提供了宝贵的基因组资源。
Hevea brasiliensis Muell. Arg, a member of the family Euphorbiaceae, is the sole natural resource exploited for commercial production of high-quality natural rubber. The properties of natural rubber latex are almost irreplaceable by synthetic counterparts for many industrial applications. A paucity of knowledge on the molecular mechanisms of rubber biosynthesis in high yield traits still persists. Here we report the comprehensive genome-wide analysis of the widely planted H. brasiliensis clone, RRIM 600. The genome was assembled based on ~155-fold combined coverage with Illumina and PacBio sequence data and has a total length of 1.55 Gb with 72.5% comprising repetitive DNA sequences. A total of 84,440 high-confidence protein-coding genes were predicted. Comparative genomic analysis revealed strong synteny between H. brasiliensis and other Euphorbiaceae genomes. Our data suggest that H. brasiliensis’s capacity to produce high levels of latex can be attributed to the expansion of rubber biosynthesis-related genes in its genome and the high expression of these genes in latex. Using cap analysis gene expression data, we illustrate the tissue-specific transcription profiles of rubber biosynthesis-related genes, revealing alternative means of transcriptional regulation. Our study adds to the understanding of H. brasiliensis biology and provides valuable genomic resources for future agronomic-related improvement of the rubber tree.
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影响因子：
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作者：
Griffiths-Jones, S;Bateman, A;Eddy, SR
通讯作者：
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影响因子：
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作者：
Benson, G
通讯作者：
Benson, G