Intrarenal distribution of the colonic H,K-ATPase mRNA in rabbit.
Intrarenal distribution of the colonic H,K-ATPase mRNA in rabbit.
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兔结肠 H,K-ATPase mRNA 的肾内分布。
DOI：
10.1046/j.1523-1755.1999.00638.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Velazquez,H
中科院分区：
文献类型：
--
作者：
Fejes-Toth,G;Naray-Fejes-Toth,A;Velazquez,H
证据表明，结肠H，K-α酶亚型在肾脏中表达，与大鼠和豚鼠HK-ATPase 2高度同源的一种基因在兔的皮质集合管中表达。本研究的目的是确定该基因是兔HKα2的同源物还是新的异构体，并确定HKα2mRNA在兔肾内的分布。方法采用5‘-RACE和染料脱氧终止剂化学方法测定兔HKα2mRNA的全长序列。用免疫组织化学方法检测肾小管上皮细胞、肾小管上皮细胞和肾小管上皮细胞中HK-α-2m RNA在肾内的分布。用荧光激活细胞分选法分离原代细胞和α-、β-嵌合细胞。采用定量逆转录聚合酶链式反应和单肾单位逆转录聚合酶链式反应检测HKα2m RNA的表达。结果获得了兔肾HKα2m RNA的全长序列。该转录本与兔远端结肠中表达的转录本完全相同。在显微解剖的肾单位节段，HK-α-2强扩增出现在CNT、Ccd和外髓集合管中，而在近曲小管、远曲小管、Think上肢和内髓集合管中未检测到信号。HK-α-2m RNA在3种细胞类型中的表达水平基本相当，且在可能与CNT细胞相对应的亚群中表达水平最高。结论兔集合管中表达的HK-α-2m RNA在大小和序列上与兔远端结肠中表达的相同。在兔肾组织中，HK-α-2m RNA选择性地表达在神经管、腔上皮细胞和外膜间充质层，3种集合管亚型均表达其m RNA。
Intrarenal distribution of the colonic H,K-ATPase mRNA in rabbit.BackgroundEvidence suggests that the colonic H,K-ATPase isoform is expressed in the kidney and that a mRNA species highly homologous to the rat and guinea pig HKα2 is expressed in the cortical collecting duct (CCD) of the rabbit. The goals of this study were to determine if this mRNA is the rabbit homologue of HKα2 or a novel isoform and to determine intrarenal distribution of the HKα2 mRNA in rabbit.Methods5′-RACE and Dye Deoxy Terminator chemistry were used to determine the full-length sequence of the rabbit HKα2 mRNA. The intrarenal distribution of HKα2 mRNA was determined in microdissected nephron segments, connecting tubule (CNT), and CCD cells isolated by immunodissection, as well as in the three cell types of the CCD. Principal cells and α- and β-intercalated cells were isolated by fluorescence-activated cell sorting. HKα2 mRNA levels were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR) or single-nephron RT-PCR (SN-RTPCR).ResultsThe full-length sequence of the rabbit kidney HKα2 mRNA was determined. This transcript is identical to the one expressed in rabbit distal colon. In microdissected nephron segments, strong HKα2 amplicons were present in the CNT, CCD, and outer medullary collecting duct (OMCD), whereas no signal was detected in the proximal tubule, distal convoluted tubule, think ascending limb, and inner medullary collecting duct. Roughly comparable levels of HKα2 mRNA were present in all three CCD cell types, and the highest levels were observed in a subpopulation most likely corresponding to CNT cells.ConclusionsThese results suggest that the HKα2 mRNA is expressed in rabbit collecting duct is identical in size and sequence to the one expressed in rabbit distal colon. HKα2 mRNA in the rabbit kidney is selectively expressed in the CNT, CCD, and OMCD, and all three collecting duct subtypes express its mRNA.
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大鼠 H -K -ATP 酶 α2 亚基的新型 N 末端剪接变体。
DOI：
10.1074/jbc.273.5.2543
发表时间：
1998
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kone,BC;Higham,SC
通讯作者：
Higham,SC
分离的主细胞和闰细胞：激素反应性和 Na -K -ATP 酶活性。
DOI：
10.1152/ajprenal.1989.256.4.f742
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
Fejes-Toth,G;Naray-Fejes-Toth,A
通讯作者：
Naray-Fejes-Toth,A
离体豚鼠结肠细胞中的 K 转运：Na(+) 独立的哇巴因敏感 K 泵的证据。
DOI：
10.1152/ajpgi.1994.266.6.g1083
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
J. D. del Castillo;M. C. Súlbaran;L. Burguillos
通讯作者：
L. Burguillos
兔远曲小管共表达 NaCl 协同转运蛋白和 11 β-羟基类固醇脱氢酶 II mRNA。
影响因子：
19.6
作者：
H. Velázquez;A. Náray;T. Silva;E. Andújar;R. Reilly;G. Desir;D. Ellison
通讯作者：
D. Ellison
皮质集合管细胞的免疫选择和培养
DOI：
10.1007/bf02388123
发表时间：
1991
期刊：
Journal of Tissue Culture Methods
影响因子：
--
作者：
A. Náray;G. Fejes
通讯作者：
G. Fejes