Regulation of tyrosinase mRNA levels in mouse melanoma cell clones by melanocyte-stimulating hormone and cyclic AMP
Regulation of tyrosinase mRNA levels in mouse melanoma cell clones by melanocyte-stimulating hormone and cyclic AMP
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黑素细胞刺激激素和环磷酸腺苷对小鼠黑色素瘤细胞克隆中酪氨酸酶 mRNA 水平的调节
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
B. Fuller
中科院分区：
文献类型：
--
作者：
G. Hoganson;F. Ledwitz‐Rigby;R. Davidson;B. Fuller
培养的小鼠黑色素瘤细胞对黑色素细胞刺激激素（MSH）有反应，表现出酪氨酸酶（黑色素合成的限速酶）的活性增加。由于这种刺激严格依赖于持续的转录和翻译，我们进行了研究，以确定MSH是否增加酪氨酸酶mRNA的水平。使用946碱基对小鼠酪氨酸酶cDNA探针，通过Northern blot分析确定MSH或二丁基cAMP处理黑色素瘤细胞中酪氨酸酶信息水平的丰度。酪氨酸酶cDNA从茶碱诱导的Cloudman黑色素瘤细胞mRNA中分离得到的λgt11表达文库中分离得到。在无色素细胞克隆（AM-7AS）和黑色素细胞克隆（MEL-11AS）中测定酪氨酸酶mRNA的丰度。黑化细胞系的酪氨酸酶活性是无色素细胞系的5倍，酪氨酸酶mRNA是无色素细胞系的10倍。添加MSH后，两种细胞系酪氨酸酶活性和mRNA均升高。无色素系经MSH处理3天后，酪氨酸酶活性增加5倍，酪氨酸酶mRNA增加2倍。用MSH处理3天后，黑色素细胞的酶活性增加3.7倍，酪氨酸酶mRNA丰度增加8倍。二丁基cAMP还能刺激酪氨酸酶活性和酪氨酸酶mRNA的积累。数据表明MSH通过cAMP作用，促进酪氨酸酶mRNA的积累。
Mouse melanoma cells in culture respond to melanocyte-stimulating hormone (MSH) by demonstrating increased activity of tyrosinase, the rate-limiting enzyme for melanin synthesis. Because this stimulation is strictly dependent upon continued transcription and translation, we have carried out studies to determine if MSH increases the level of tyrosinase mRNA. The abundance of tyrosinase message levels in melanoma cells treated with either MSH or dibutyryl cAMP was determined by Northern blot analysis utilizing a 946 base pair mouse tyrosinase cDNA probe. The tyrosinase cDNA was isolated from a λgt11 expression library generated from mRNA isolated from theopylline-induced Cloudman melanoma cells. The abundance of tyrosinase mRNA was determined in an amelanotic cell clone (AM-7AS) and a melanotic cell clone (MEL-11AS). The melanotic cell line had five times as much tyrosinase activity and almost 10 times more tyrosinase mRNA than the amelanotic line. Tyrosinase activity and mRNA increased in both cell lines after MSH addition. The amelanotic line treated with MSH for three days showed a fivefold increase in tyrosinase activity and a twofold increase in tyrosinase mRNA. The melanotic cell line treated with MSH for three days showed a 3.7-fold increase in enzyme activity and an eightfold increase in the abundance of tyrosinase mRNA. Dibutyryl cAMP also stimulated tyrosinase activity and the accumulation of tyrosinase mRNA. The data suggest that MSH, acting through cAMP, promotes an accumulation of tyrosinase mRNA.
DOI：
10.1016/s0006-291x(88)81370-6
发表时间：
1988-06-30
影响因子：
3.1
作者：
KWON, BS;WAKULCHIK, M;KESTLER, D
通讯作者：
KESTLER, D
DOI：
10.1073/pnas.84.21.7473
发表时间：
1987-11-01
影响因子：
11.1
作者：
KWON, BS;HAQ, AK;HALABAN, R
通讯作者：
HALABAN, R
Cloudman S-91 小鼠黑色素瘤细胞培养物中酪氨酸酶的α-黑色素细胞刺激激素调节。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fuller,BB;Lunsford,JB;Iman,DS
通讯作者：
Iman,DS