Features of Vernix Caseosa Cells
Features of Vernix Caseosa Cells
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胎脂细胞的特点
DOI：
--
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发表时间：
1988
影响因子：
2
通讯作者：
A.H. Papaloucas
中科院分区：
文献类型：
--
作者：
T. Agorastos;G. Hollweg;E. Grussendorf;A.H. Papaloucas
为了收集更多关于皮脂（VC）的信息，我们研究了VC细胞的一些组织化学、超微结构和免疫学特性。用组织化学和光镜观察VC细胞中酸性和碱性磷酸酶的活性，这些酶在羊水中的活性在足月时显著增加。酸性磷酸酶活性以胞浆内颗粒或细胞间无定形物质的形式强烈存在；碱性磷酸酶活性完全不存在。采用扫描电镜和透射电镜观察VC细胞的超微结构形态。在VC角化细胞的个别表面模式中可以显示出显著的差异。透射电镜显示角化不同阶段的不规则扁平细胞。超微结构结果证实了存在于单个VC细胞之间的差异性。最后，冷冻VC涂片的免疫荧光染色试验显示，只有免疫球蛋白G偶联物在VC细胞抗原位点产生强烈的阳性反应。本研究的特别发现是VC细胞的表面形态和细胞质结构的多态性，以及细胞内和细胞间酸性磷酸酶活性的不均匀性。所有这些都表明单个VC细胞在角化和脱屑活动方面的状态是不相似的。
In an attempt to collect more information about the features of the vernix caseosa (VC), a relatively unstudied material, some of the histochemical, ultrastructural, and immunological characteristics of VC cells have been investigated. Histochemistry and light microscopy was used to demonstrate the activity of acid and alkaline phosphatase in VC cells, enzymes with a marked increase in activity in the amniotic fluid toward term. Acid phosphatase activity was strongly present either as intracytoplasmic granules or as amorphous material between the cells; alkaline phosphatase activity was absolutely nonexistent. The ultrastructural morphology of the VC cells was analyzed by scanning and transmission electron microscopy (TEM). Significant differences can be demonstrated in the individual surface patterns of the VC keratinocytes. TEM showed irregularly flattened cells in various stages of keratinization. The ultrastructural findings confirm the dissimilarity, which exists between the individual VC cells. Finally, immunofluorescent staining tests of frozen VC smears showed that only immunoglobulin G conjugate gives strong positive reaction at the antigen sites of the VC cells. The special finding in this study is the polymorph appearance of the surface pattern and the cytoplasma structure of the VC cells, as well as the lack of uniform appearance of the acid phosphatase activity in and between the cells. All these suggest that the status of the individual VC cell is not similar in regard to their keratinization and desquamation activities.
酪脂蜡酯脂肪酸组成的变化及其与皮脂腺活性的可能关系。
DOI：
10.1111/1523-1747.ep12507228
发表时间：
1982
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Stewart,ME;Quinn,MA;Downing,DT
通讯作者：
Downing,DT
表皮酸性水解酶：亚细胞定位及其与角化的关系。
DOI：
10.1111/1523-1747.ep12555534
发表时间：
1983
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Freinkel,RK;Traczyk,TN
通讯作者：
Traczyk,TN