Irisin has no effect on lipolysis in 3T3-L1 adipocytes or fatty acid metabolism in HepG2 hepatocytes
Irisin has no effect on lipolysis in 3T3-L1 adipocytes or fatty acid metabolism in HepG2 hepatocytes
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Irisin 对 3T3-L1 脂肪细胞的脂肪分解或 HepG2 肝细胞的脂肪酸代谢没有影响
DOI：
10.1007/s12020-014-0458-9
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发表时间：
2015-05
期刊：
影响因子：
3.7
通讯作者：
Chen Li
中科院分区：
文献类型：
--
作者：
Wang Chuan;Wang Lingshu;Li Wenjuan;Yan Fei;Tian Meng;Wu Chuanlong;Qi Lin;Wang Xuping;Song Jun;Hou Xinguo;Chen Li
鸢尾素是一种新发现的肌肉因子，负责白色或米色脂肪细胞的褐化，据报道，糖尿病患者的鸢尾素水平较低，并与肥胖、血清甘油三酯（TG）水平和肝内TG水平相关。我们想知道鸢尾素是否可以直接影响脂肪细胞和肝细胞的脂肪酸和TG代谢。我们检测了不同浓度的鸢尾素对成熟3T3-L1脂肪细胞中脂肪分解（根据油红O染色、游离脂肪酸释放和甘油释放）、HSL和ATGL蛋白表达以及其他脂质相关基因（UCP-1、PPARγ、FABP-4、HSL、ATGL、PPARα和CPT-1） mRNA表达的影响。以及生理或高血糖条件下HepG2肝细胞脂肪酸合成（SREBP-1C和FAS）和β-氧化（PPARα和CPT-1）相关基因的mRNA水平。我们的研究结果显示，尽管鸢尾素显著增加了UCP-1和PPARα的mRNA水平，但它对成熟3T3-L1脂肪细胞中脂解、HSL或ATGL蛋白水平或其他脂质相关基因的mRNA表达没有可检测到的影响。在HepG2肝细胞中，高糖诱导SREBP-1C和FAS上调，PPARα下调；然而，在生理或高血糖条件下，鸢尾素对基因表达没有显著影响。因此，我们得出结论，鸢尾素对3T3-L1脂肪细胞的脂肪分解或HepG2肝细胞的脂肪酸代谢没有显著的直接影响。
Irisin, a newly identified myokine responsible for browning of white or beige adipocytes, has been reported to be present at reduced levels in diabetic patients and associated with obesity, serum triglyceride (TG) levels, and intrahepatic TG levels. We wondered whether irisin could directly affect fatty acid and TG metabolism in adipocytes and hepatocytes. We examined the effects of various concentrations of irisin on lipolysis (according to Oil Red O staining, free fatty acid release, and glycerol release), protein expression of HSL and ATGL, and mRNA expression of other lipid-related genes (UCP-1, PPARγ, FABP-4, HSL, ATGL, PPARα, and CPT-1) in mature 3T3-L1 adipocytes, as well as mRNA levels of genes involved in the synthesis (SREBP-1C and FAS) and β-oxidation (PPARα and CPT-1) of fatty acids in HepG2 hepatocytes under physiological or hyperglycemic conditions. Our results revealed that although irisin significantly increased the mRNA levels of UCP-1 and PPARα, it failed to show detectable effects on lipolysis, HSL or ATGL protein levels, or the mRNA expression of other lipid-related genes in mature 3T3-L1 adipocytes. In HepG2 hepatocytes, high glucose induced the upregulation of SREBP-1C and FAS and the downregulation of PPARα; however, no significant effect of irisin on gene expression was observed under either physiological or hyperglycemic conditions. We therefore conclude that irisin has no significant direct effect on lipolysis in 3T3-L1 adipocytes or on fatty acid metabolism in HepG2 hepatocytes.
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影响因子：
5.1
作者：
Choi, Yeon-Kyung;Kim, Mi-Kyung;Park, Keun-Gyu
通讯作者：
Park, Keun-Gyu
影响因子：
5.4
作者：
Yang, Junpeng;Ren, Jianmin;Chen, Li
通讯作者：
Chen, Li
影响因子：
5.8
作者：
Park, Kyung Hee;Zaichenko, Lesya;Mantzoros, Christos S.
通讯作者：
Mantzoros, Christos S.
影响因子：
9.8
作者：
Polyzos, Stergios A.;Kountouras, Jannis;Mantzoros, Christos S.
通讯作者：
Mantzoros, Christos S.
影响因子：
40.5
作者：
A. Cunha
通讯作者：
A. Cunha