Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout.
Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout.
复制标题
DOI：
10.3390/toxins15040273
复制
发表时间：
2023-04-06
期刊：
影响因子：
4.2
通讯作者：
You S
中科院分区：
文献类型：
--
作者：
Xiong L;Liu Z;Li J;Yao S;Li Z;Chen X;Shen L;Zhang Z;Li Y;Hou Q;Zhang Y;You M;Yuchi Z;You S
Many insects, including the Plutella xylostella (L.), have developed varying degrees of resistance to many insecticides, including Bacillus thuringiensis (Bt) toxins, the bioinsecticides derived from Bt. The polycalin protein is one of the potential receptors for Bt toxins, and previous studies have confirmed that the Cry1Ac toxin can bind to the polycalin protein of P. xylostella, but whether polycalin is associated with the resistance of Bt toxins remains controversial. In this study, we compared the midgut of larvae from Cry1Ac-susceptible and -resistant strains, and found that the expression of the Pxpolycalin gene was largely reduced in the midgut of the resistant strains. Moreover, the spatial and temporal expression patterns of Pxpolycalin showed that it was mainly expressed in the larval stage and midgut tissue. However, genetic linkage experiments showed that the Pxpolycalin gene and its transcript level were not linked to Cry1Ac resistance, whereas both the PxABCC2 gene and its transcript levels were linked to Cry1Ac resistance. The larvae fed on a diet containing the Cry1Ac toxin showed no significant change in the expression of the Pxpolycalin gene in a short term. Furthermore, the knockout of polycalin and ATP-binding cassette transporter subfamily C2 (ABCC2) genes separately by CRISPR/Cas9 technology resulted in resistance to decreased susceptibility to Cry1Ac toxin. Our results provide new insights into the potential role of polycalin and ABCC2 proteins in Cry1Ac resistance and the mechanism underlying the resistance of insects to Bt toxins.
登录
查看更多内容
棉铃虫钙粘蛋白片段与 Cry1Ac 在不同细胞和昆虫中的协同选择
DOI：
10.1016/j.ijbiomac.2020.08.175
发表时间：
2020-12-01
影响因子：
8.2
作者：
Hao, Jia;Gao, Meijing;Liu, Xianjin
通讯作者：
Liu, Xianjin
影响因子：
3
作者：
Wang J;Yang X;He H;Chen J;Liu Y;Huang W;Ou L;Yang Z;Guan X;Zhang L;Wu S
通讯作者：
Wu S
影响因子：
4.5
作者：
Gahan LJ;Pauchet Y;Vogel H;Heckel DG
通讯作者：
Heckel DG
影响因子：
4.6
作者：
Guo Z;Kang S;Zhu X;Xia J;Wu Q;Wang S;Xie W;Zhang Y
通讯作者：
Zhang Y
DOI：
10.1016/j.ijbiomac.2019.12.063
发表时间：
2020-03-01
影响因子：
8.2
作者：
Gao, Meijing;Hu, Xiaodan;Liu, Xianjin
通讯作者：
Liu, Xianjin