Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers
Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers
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在大肠杆菌中设计合成由偶数链和奇数链单体组成的中链长度聚羟基脂肪酸酯的途径
DOI：
10.1186/s12934-019-1186-x
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发表时间：
2019-08
影响因子：
6.4
通讯作者：
Qi Qingsheng
中科院分区：
文献类型：
--
作者：
Zhuang Qianqian;Qi Qingsheng
背景：中链聚羟基脂肪酸酯（mcl-PHAs）是由C6 ~ C14不同链长的单体组成，具有优良的上级物理机械性能，在可持续发展和环境友好的生物材料领域有着广泛的应用。我们在大肠杆菌中设计了一条反向脂肪酸β-氧化途径，可以直接从葡萄糖合成mcl-PHA，并获得了高产率。然而，在生物合成聚合物中只有偶链单体。结果：在本研究中，将丙酸同化和代谢途径整合到反向脂肪酸β-氧化中，以生产由偶数和奇数单体组成的mcl-PHA。随着丙酸酯添加量的增加，mcl-PHA中奇数单体的含量增加。在进一步缺失丙酮酸氧化酶（PoxB）和丙酮酸甲酸裂解酶（PflB）后，代谢工程化的底盘E.在大肠杆菌LZ 08中，分别过表达了真养罗尔斯通氏菌H16的prpP和prpE基因pQQ 05和pZQ 06，获得了含7 ~ 13个碳原子的奇链单体（约20.03mol%）的mcl-PHA，产率为6.23wt%。杆菌本研究在E.大肠杆菌的摇瓶发酵水平。宿主菌株和途径酶的持续工程改造将最终导致基于市场需求的奇数链单体的更经济的生产。该合成途径可以为生产其他增值化学品和生物材料提供有前途的平台，这些化学品和生物材料使用乙酰辅酶A和丙酰辅酶A作为通用前体，并且可以作为智能细胞工厂扩展到其他微生物。
Background:Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their superior physical and mechanical properties. We engineered a reversed fatty acid β-oxidation pathway in Escherichia coli that can synthesize mcl-PHA directly from glucose and achieved high yield. However, there were only even-chain monomers in the biosynthetic polymers. The need for mcl-PHA harboring both even- and odd-chain monomers with better and wider utility impels us to develop the biosynthetic routes for the production of the novel and unnatural mcl-PHA through rewiring the basic metabolism.Results:In the present study, a propionate assimilation and metabolic route was integrated into the reversed fatty acid β-oxidation in order to produce mcl-PHA consisting of both even- and odd-numbered monomers. The content of odd-numbered monomers in mcl-PHA was improved with the increased propionate addition. After further deletion of pyruvate oxidase (PoxB) and pyruvate formate-lyase (PflB), the metabolically engineered chassis E. coli LZ08 harboring pQQ05 and pZQ06 (overexpression of prpP and prpE genes from Ralstonia eutropha H16) innovatively accumulated 6.23 wt% mcl-PHA containing odd-chain monomers ranging from 7 to 13 carbon atoms about 20.03 mol%.Conclusions:This is the first successful report on production of mcl-PHA harboring both even- and odd-chain monomers (C6-C14) synthesized from glucose and propionate in recombinant E. coli. This present study achieved the highest yield of de novo production of mcl-PHA containing odd-numbered monomers in E. coli at shake-flask fermentation level. Continued engineering of host strains and pathway enzymes will ultimately lead to more economical production of odd-chain monomers based on market demand. The synthetic pathway can provide a promising platform for production of other value-added chemicals and biomaterials that use acetyl-CoA and propionyl-CoA as versatile precursors and can be extended to other microorganisms as intelligent cell factories.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
2.1
作者：
Qi, QS;Rehm, BHA;Steinbüchel, A
通讯作者：
Steinbüchel, A
影响因子：
8.4
作者：
Agnew, Daniel E.;Stevermer, Amanda K.;Youngquist, J. Tyler;Pfleger, Brian F.
通讯作者：
Pfleger, Brian F.
诱导竹子真菌 Shiraia sp 产生苝醌。
影响因子：
6.4
作者：
Ma, Yan Jun;Zheng, Li Ping;Wang, Jian Wen
通讯作者：
Wang, Jian Wen
影响因子：
5
作者：
Springer-Verlag;Berlin Heidelberg
通讯作者：
Springer-Verlag;Berlin Heidelberg