Tomographic Imaging and Localization of Nanoparticles in Tissue Using Surface-Enhanced Spatially Offset Raman Spectroscopy.
Tomographic Imaging and Localization of Nanoparticles in Tissue Using Surface-Enhanced Spatially Offset Raman Spectroscopy.
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DOI：
10.1021/acsami.2c05611
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发表时间：
2022-07-20
影响因子：
9.5
通讯作者：
Faulds, Karen
中科院分区：
文献类型：
--
作者：
Berry, Matthew E.;McCabe, Samantha M.;Sloan-Dennison, Sian;Laing, Stacey;Shand, Neil C.;Graham, Duncan;Faulds, Karen
对于表面增强空间偏移拉曼光谱（SESORS）成像和在临床环境中实现其用于体内诊断目的至关重要的一个基本问题是SESORS图像是否可以用于确定组织内对象的确切位置？为了解决这个问题，多个实验因素有关的光学设置在成像实验中使用内部建立的点收集为基础的空间偏移拉曼光谱（SORS）系统进行了研究，以确定那些关键的三维（3D）定位能力的SESORS。在这里，我们报告的影响的空间偏移量和几何定位纳米粒子（NP）与二氧化硅粉末混合作为成像目标，通过组织和大纲实验技术，允许正确的解释SESORS图像，以确定正确的位置的NP在二维x，y-成像平面的深度。更具体地，提出了“线性偏移引起的图像拖曳”的效果，其指的是由线性偏移的幅度和方向引起的SESORS图像中的空间失真，并且突出了在成像期间对环形SORS收集几何结构的需要，以中和这些不对称效果。此外，在这些原则的基础上，引入了“比例SESORS成像”的概念，用于在三维空间中定位埋藏的包裹体。总之，这些原则是至关重要的，在开发一种方法，在三维空间中的表面增强拉曼散射活性夹杂物的位置。该方法利用空间偏移的幅度、探测深度以及NP和组织拉曼强度的比率分析之间的关系，以首次最终对埋在3D模型内的不同深度处的两种不同NP风味进行成像和空间区分。这项研究演示了如何使用SESORS准确识别组织中深度的多个对象及其位置，这解决了将SESORS更接近生物医学应用的关键能力。
A fundamental question crucial to surface-enhanced spatially offset Raman spectroscopy (SESORS) imaging and implementing it in a clinical setting for in vivo diagnostic purposes is whether a SESORS image can be used to determine the exact location of an object within tissue? To address this question, multiple experimental factors pertaining to the optical setup in imaging experiments using an in-house-built point-collection-based spatially offset Raman spectroscopy (SORS) system were investigated to determine those critical to the three-dimensional (3D) positioning capability of SESORS. Here, we report the effects of the spatial offset magnitude and geometry on locating nanoparticles (NPs) mixed with silica powder as an imaging target through tissue and outline experimental techniques to allow for the correct interpretation of SESORS images to ascertain the correct location of NPs in the two-dimensional x, y-imaging plane at depth. More specifically, the effect of “linear offset-induced image drag” is presented, which refers to a spatial distortion in SESORS images caused by the magnitude and direction of the linear offset and highlight the need for an annular SORS collection geometry during imaging to neutralize these asymmetric effects. Additionally, building on these principles, the concept of “ratiometric SESORS imaging” is introduced for the location of buried inclusions in three dimensions. Together these principles are vital in developing a methodology for the location of surface-enhanced Raman scattering-active inclusions in three dimensions. This approach utilizes the relationship between the magnitude of the spatial offset, the probed depth, and ratiometric analysis of the NP and tissue Raman intensities to ultimately image and spatially discriminate between two distinct NP flavors buried at different depths within a 3D model for the first time. This research demonstrates how to accurately identify multiple objects at depth in tissue and their location using SESORS which addresses a key capability in moving SESORS closer to use in biomedical applications.
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DOI：
10.1039/d1an00865j
发表时间：
2021-10-11
期刊：
The Analyst
影响因子：
--
作者：
Berry ME;Kearns H;Graham D;Faulds K
通讯作者：
Faulds K
影响因子：
8.4
作者：
Nicolson F;Jamieson LE;Mabbott S;Plakas K;Shand NC;Detty MR;Graham D;Faulds K
通讯作者：
Faulds K
影响因子：
4.2
作者：
Matousek, Pavel;Stone, Nicholas
通讯作者：
Stone, Nicholas
影响因子：
8.4
作者：
Kapara A;Brunton V;Graham D;Faulds K
通讯作者：
Faulds K
影响因子：
7.4
作者：
Botteon, Alessandra;Conti, Claudia;Matousek, Pavel
通讯作者：
Matousek, Pavel