Through tissue imaging of a live breast cancer tumour model using handheld surface enhanced spatially offset resonance Raman spectroscopy (SESORRS).
Through tissue imaging of a live breast cancer tumour model using handheld surface enhanced spatially offset resonance Raman spectroscopy (SESORRS).
复制标题
通过使用手持式表面增强空间偏移共振拉曼光谱法（SESORRS）对活体乳腺癌肿瘤模型进行组织成像。
DOI：
10.1039/c8sc00994e
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发表时间：
2018-04-21
期刊：
影响因子：
8.4
通讯作者：
Faulds K
中科院分区：
文献类型：
--
作者：
Nicolson F;Jamieson LE;Mabbott S;Plakas K;Shand NC;Detty MR;Graham D;Faulds K
使用SESORRS通过15 mm组织检测活体3D肿瘤模型。为了提高患者的生存率并减少不必要的和创伤性的活组织检查的量，对癌性肿瘤的非侵入性检测是迫切需要的。多细胞肿瘤球状体（MTS）可用作离体癌症肿瘤模型，以通过增强的渗透性和保留（EPR）效应来模拟体内纳米颗粒（NP）摄取。表面增强空间偏移拉曼光谱（SESORS）结合了表面增强拉曼光谱（Sers）和空间偏移拉曼光谱（SORS），以在更大的次表面水平上产生增强的拉曼信号。通过利用具有与激光频率共振的电子跃迁的报告物，表面增强共振拉曼散射（SERRS）在拉曼信号中产生甚至更大的增强。使用具有背散射光学器件的手持式SORS光谱仪，我们证明了通过15 mm的猪组织检测含有SERRS活性NP的活乳腺癌3D MTS。使用假彩色2D热强度图来确定肿瘤模型位置。此外，我们证明了跟踪SERRS活性纳米粒子通过猪组织的深度高达25毫米。这是前所未有的性能是由于使用的红移硫属吡喃基拉曼记者证明的新技术的表面增强空间偏移共振拉曼光谱（SESORRS）的第一次。我们的研究结果表明，在通过组织深度检测来自肿瘤模型的振动指纹的能力方面迈出了重要的一步。这种方法为将NP转化为基于这种新的化学测量原理的非侵入性疾病诊断的临床应用提供了重要的前景。
Detection of a live 3D tumour model through 15 mm of tissue using SESORRS. In order to improve patient survival and reduce the amount of unnecessary and traumatic biopsies, non-invasive detection of cancerous tumours is of imperative and urgent need. Multicellular tumour spheroids (MTS) can be used as an ex vivo cancer tumour model, to model in vivo nanoparticle (NP) uptake by the enhanced permeability and retention (EPR) effect. Surface enhanced spatially offset Raman spectroscopy (SESORS) combines both surface enhanced Raman spectroscopy (SERS) and spatially offset Raman spectroscopy (SORS) to yield enhanced Raman signals at much greater sub-surface levels. By utilizing a reporter that has an electronic transition in resonance with the laser frequency, surface enhanced resonance Raman scattering (SERRS) yields even greater enhancement in Raman signal. Using a handheld SORS spectrometer with back scattering optics, we demonstrate the detection of live breast cancer 3D MTS containing SERRS active NPs through 15 mm of porcine tissue. False color 2D heat intensity maps were used to determine tumour model location. In addition, we demonstrate the tracking of SERRS-active NPs through porcine tissue to depths of up to 25 mm. This unprecedented performance is due to the use of red-shifted chalcogenpyrylium-based Raman reporters to demonstrate the novel technique of surface enhanced spatially offset resonance Raman spectroscopy (SESORRS) for the first time. Our results demonstrate a significant step forward in the ability to detect vibrational fingerprints from a tumour model at depth through tissue. Such an approach offers significant promise for the translation of NPs into clinical applications for non-invasive disease diagnostics based on this new chemical principle of measurement.
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影响因子：
16.6
作者：
Harmsen, Stefan;Bedics, Matthew A.;Wall, Matthew A.;Huang, Ruimin;Detty, Michael R.;Kircher, Moritz F.
通讯作者：
Kircher, Moritz F.
影响因子：
4.6
作者：
Ellis DI;Eccles R;Xu Y;Griffen J;Muhamadali H;Matousek P;Goodall I;Goodacre R
通讯作者：
Goodacre R
影响因子：
4.2
作者：
Kearns, Hayleigh;Bedics, Matthew A.;Graham, Duncan
通讯作者：
Graham, Duncan
影响因子：
7.4
作者：
Ma, Ke;Yuen, Jonathan M.;Shah, Nilam C.;Walsh, Joseph T., Jr.;Glucksberg, Matthew R.;Van Duyne, Richard P.
通讯作者：
Van Duyne, Richard P.
影响因子：
9.5
作者：
Asiala, Steven M.;Shand, Neil C.;Graham, Duncan
通讯作者：
Graham, Duncan