Changes in circulating microRNA levels associated with prostate cancer.
Changes in circulating microRNA levels associated with prostate cancer.
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DOI：
10.1038/bjc.2011.595
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发表时间：
2012-02-14
影响因子：
8.8
通讯作者：
Hamdy, F. C.
中科院分区：
文献类型：
--
作者：
Bryant, R. J.;Pawlowski, T.;Catto, J. W. F.;Marsden, G.;Vessella, R. L.;Rhees, B.;Kuslich, C.;Visakorpi, T.;Hamdy, F. C.
本研究的目的是调查循环microRNA（miR）的变化代表前列腺癌诊断，分期和预后预测的潜在有用的生物标志物的假设。使用78名前列腺癌患者和28名正常对照个体的血浆来源的循环微泡对742个miR进行实时聚合酶链反应分析，以鉴定差异定量的miR。与对照组相比，在前列腺癌患者中共对12个miR进行了差异定量，包括9个在无转移的患者中。总而言之，与无转移的前列腺癌患者相比，有转移的前列腺癌患者中存在11种miR，其含量显着增加。miR-141和miR-375与转移性前列腺癌的相关性在根治性前列腺切除术后复发或非复发疾病的单独患者队列中使用血清来源的外泌体和微囊泡证实。对尿样中五种选定的miR的分析发现，与对照组相比，前列腺癌男性尿液中的miR-107和miR-574- 3 p定量浓度显著更高。这些观察结果表明，可以通过分析各种体液来确定前列腺癌患者中miR浓度的变化。此外，循环miR可用于诊断和分期前列腺癌。
The aim of this study was to investigate the hypothesis that changes in circulating microRNAs (miRs) represent potentially useful biomarkers for the diagnosis, staging and prediction of outcome in prostate cancer. Real-time polymerase chain reaction analysis of 742 miRs was performed using plasma-derived circulating microvesicles of 78 prostate cancer patients and 28 normal control individuals to identify differentially quantified miRs. A total of 12 miRs were differentially quantified in prostate cancer patients compared with controls, including 9 in patients without metastases. In all, 11 miRs were present in significantly greater amounts in prostate cancer patients with metastases compared with those without metastases. The association of miR-141 and miR-375 with metastatic prostate cancer was confirmed using serum-derived exosomes and microvesicles in a separate cohort of patients with recurrent or non-recurrent disease following radical prostatectomy. An analysis of five selected miRs in urine samples found that miR-107 and miR-574-3p were quantified at significantly higher concentrations in the urine of men with prostate cancer compared with controls. These observations suggest that changes in miR concentration in prostate cancer patients may be identified by analysing various body fluids. Moreover, circulating miRs may be used to diagnose and stage prostate cancer.
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影响因子：
8.4
作者：
Ciatto, S;Zappa, M;Gervasi, G
通讯作者：
Gervasi, G
影响因子：
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Coltman, CA
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作者：
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通讯作者：
Schalken, JA