Advances in Brief Hyaluronan-CD 44 s Signaling Regulates Matrix Metalloproteinase-2 Secretion in a Human Lung Carcinoma Cell Line QG 901
Advances in Brief Hyaluronan-CD 44 s Signaling Regulates Matrix Metalloproteinase-2 Secretion in a Human Lung Carcinoma Cell Line QG 901
复制标题
透明质酸-CD 44 s 信号传导调节人肺癌细胞系 QG 901 中基质金属蛋白酶-2 分泌的简要进展
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
M. Hamaguchi
中科院分区：
文献类型：
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作者：
Yanying Zhang;A. Thant;K. Machida;Y. Ichigotani;Y. Naito;Y. Hiraiwa;T. Senga;Yasuyoshi Sohara;S. Matsuda;M. Hamaguchi
We investigated the production of matrix metalloproteinase (MMP) by hyaluronan (HA) stimulation in a human cancer cell line, QG90, that expresses a large amount of CD44s, a HA receptor. Treatment of QG90 with HA strongly activated MMP-2 secretion in a timeand dose-dependent manner. We found that expression of antisense CD44s in QG90 cells substantially inhibited the HA-dependent secretion of MMP-2, whereas overexpression of full-length CD44s augmented the HA-dependent secretion of MMP-2. In addition, pretreatment of cells with the neutralizing anti-CD44 antibody significantly inhibited both the HA-dependent MMP-2 secretion and the HA-dependent activation of mitogen-activated protein kinase in a dose-dependent manner. Similarly, treatment of cells with a Ras farnesyltransferase inhibitor, manumycin A, strongly inhibited the HA-dependent MMP-2 secretion. Moreover, in vitro invasiveness of QG90 and its activation by HA were clearly suppressed by the expression of antisense CD44s. In addition, treatment of cells with anti-CD44, a mitogen-activated protein/extracellular signal-regulated kinase kinase 1 inhibitor, PD98059, or phosphatidylinositol 3 -kinase inhibitors, wortmannin and LY294002, effectively blocked the HA-dependent activation of the invasiveness. In contrast, overexpression of full-length CD44 substantially activated the invasiveness of QG90. Taken together, HA-CD44s signaling plays a key role in the HA-dependent secretion of MMP-2 and, hence, in the invasiveness of QG90 cells.
影响因子：
11.2
作者：
Stephen A. Cannistra;G. Kansas;J. Niloff;B. DeFranzo;Young Ho Kim;Christian H. Ottensmeier
通讯作者：
Stephen A. Cannistra;G. Kansas;J. Niloff;B. DeFranzo;Young Ho Kim;Christian H. Ottensmeier
影响因子：
5.7
作者：
Hompland, Tord;Lund, Kjersti V.;Rofstad, Einar K.
通讯作者：
Rofstad, Einar K.