Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools.
Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools.
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DOI：
10.1038/s41598-017-04657-x
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发表时间：
2017-07-12
影响因子：
4.6
通讯作者：
Kubo F
中科院分区：
文献类型：
--
作者：
Förster D;Arnold-Ammer I;Laurell E;Barker AJ;Fernandes AM;Finger-Baier K;Filosa A;Helmbrecht TO;Kölsch Y;Kühn E;Robles E;Slanchev K;Thiele TR;Baier H;Kubo F
从基因上获取小的、可复制的神经元集合是理解大脑功能布线的关键。在这里，我们报告了一个新的Gal 4和Cre-driver斑马鱼神经生物学资源的生成。候选基因，包括细胞类型特异性转录因子，神经递质合成酶和神经肽，根据其在不同脑区的小而独特的神经元亚群中的表达模式进行选择。BAC重组工程，然后是Tol 2转基因，用于产生驱动系，其以在很大但可变的程度上概括内源基因表达的模式标记神经元群体。我们使用图像配准来表征、比较和数字化标记来自我们新生成的转基因品系的图案。这种分析揭示了高度限制和相互排斥的组织分布，具有惊人的分层大脑区域，如顶盖或菱脑的分辨率。我们进一步表明，Gal 4和Cre转基因的组合允许在两个驱动程序的表达重叠的区域中交叉表达荧光报告基因。总而言之，我们的研究为斑马鱼特定神经回路的功能研究提供了新的工具。
Genetic access to small, reproducible sets of neurons is key to an understanding of the functional wiring of the brain. Here we report the generation of a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neurotransmitter-synthesizing enzymes and neuropeptides, were selected according to their expression patterns in small and unique subsets of neurons from diverse brain regions. BAC recombineering, followed by Tol2 transgenesis, was used to generate driver lines that label neuronal populations in patterns that, to a large but variable extent, recapitulate the endogenous gene expression. We used image registration to characterize, compare, and digitally superimpose the labeling patterns from our newly generated transgenic lines. This analysis revealed highly restricted and mutually exclusive tissue distributions, with striking resolution of layered brain regions such as the tectum or the rhombencephalon. We further show that a combination of Gal4 and Cre transgenes allows intersectional expression of a fluorescent reporter in regions where the expression of the two drivers overlaps. Taken together, our study offers new tools for functional studies of specific neural circuits in zebrafish.
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影响因子：
7
作者：
Auer TO;Duroure K;De Cian A;Concordet JP;Del Bene F
通讯作者：
Del Bene F
影响因子：
4.6
作者：
Hisano Y;Sakuma T;Nakade S;Ohga R;Ota S;Okamoto H;Yamamoto T;Kawahara A
通讯作者：
Kawahara A
DOI：
10.1073/pnas.1012185108
发表时间：
2011-01-18
影响因子：
11.1
作者：
Kinkhabwala, Amina;Riley, Michael;Fetcho, Joseph
通讯作者：
Fetcho, Joseph
影响因子：
2.7
作者：
Akitake, Courtney M.;Macurak, Michelle;Goll, Mary G.
通讯作者：
Goll, Mary G.
影响因子：
2.5
作者：
Fujimoto, Esther;Gaynes, Brooke;Brimley, Cameron J.;Chien, Chi-Bin;Bonkowsky, Joshua L.
通讯作者：
Bonkowsky, Joshua L.