A perspective on microarrays: current applications, pitfalls, and potential uses.
A perspective on microarrays: current applications, pitfalls, and potential uses.
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DOI：
10.1186/1475-2859-6-4
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发表时间：
2007-01-25
影响因子：
6.4
通讯作者：
Shiloach J
中科院分区：
文献类型：
--
作者：
Jaluria P;Konstantopoulos K;Betenbaugh M;Shiloach J
随着机器人技术、计算能力和高质量玻片制造的进步，以及公共数据库中可获得的基因组信息的增加，微阵列技术越来越多地用于世界各地的实验室。事实上，毒理学、进化生物学、药物开发和生产、疾病表征、诊断开发、细胞生理学和应激反应以及法医学等领域都受益于它的使用。然而，对于许多不熟悉微阵列的研究人员来说，当前的文章和评论往往既不涉及技术背后的基本原理，也不涉及正确的实验设计。虽然微阵列技术在概念上相对简单，但其实践确实需要仔细规划和详细了解固有的局限性。如果没有这些考虑，就很难从微阵列数据中确定有价值的信息。因此，本文旨在概述微阵列技术的关键特征，特别注意最近出版物中概述的当前应用，实验设计，统计方法和潜在用途。此外，这项审查并不是全面的，而是实质性的；强调重要的概念和详细步骤必要进行和解释微阵列实验。总的来说，本文中包含的信息将突出微阵列技术的多功能性，并提供未来可能持有的一瞥。
With advances in robotics, computational capabilities, and the fabrication of high quality glass slides coinciding with increased genomic information being available on public databases, microarray technology is increasingly being used in laboratories around the world. In fact, fields as varied as: toxicology, evolutionary biology, drug development and production, disease characterization, diagnostics development, cellular physiology and stress responses, and forensics have benefiting from its use. However, for many researchers not familiar with microarrays, current articles and reviews often address neither the fundamental principles behind the technology nor the proper designing of experiments. Although, microarray technology is relatively simple, conceptually, its practice does require careful planning and detailed understanding of the limitations inherently present. Without these considerations, it can be exceedingly difficult to ascertain valuable information from microarray data. Therefore, this text aims to outline key features in microarray technology, paying particular attention to current applications as outlined in recent publications, experimental design, statistical methods, and potential uses. Furthermore, this review is not meant to be comprehensive, but rather substantive; highlighting important concepts and detailing steps necessary to conduct and interpret microarray experiments. Collectively, the information included in this text will highlight the versatility of microarray technology and provide a glimpse of what the future may hold.
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