Construction of papaya male and female BAC libraries and application in physical mapping of the sex chromosomes.
Construction of papaya male and female BAC libraries and application in physical mapping of the sex chromosomes.
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DOI：
10.1155/2011/929472
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发表时间：
2011
影响因子：
--
通讯作者：
Ming R
中科院分区：
文献类型：
--
作者：
Gschwend AR;Yu Q;Moore P;Saski C;Chen C;Wang J;Na JK;Ming R
木瓜是热带地区的主要水果作物，最近才进化出性染色体。为了对木瓜性染色体进行测序，我们利用木瓜雄性和雌性基因组 DNA 构建了两个细菌人工染色体 (BAC) 文库。雌性 BAC 文库使用限制性内切酶 BstY I 构建，由 36,864 个克隆组成，平均插入片段大小为 104kb，提供 10.3 倍的基因组当量。雄性 BAC 文库使用限制性内切酶 EcoR I 构建，由 55,296 个克隆组成，平均插入片段大小为 101 kb，提供 15.0 倍的基因组当量。雄性BAC文库用于构建雄性Y染色体男性特异性区域(MSY)的物理图谱，以及填补空白和扩展Yh染色体雌雄同体特异性区域(HSY)的物理图谱和X染色体物理图谱。女性BAC文库用于扩大X物理图谱差距。 MSY、HSY 和 X 物理图谱为研究染色体重排、Y 染色体变性和木瓜新生性染色体的剂量补偿提供了独特的机会。
Papaya is a major fruit crop in the tropics and has recently evolved sex chromosomes. Towards sequencing the papaya sex chromosomes, two bacterial artificial chromosome (BAC) libraries were constructed from papaya male and female genomic DNA. The female BAC library was constructed using restriction enzyme BstY I and consists of 36,864 clones with an average insert size of 104 kb, providing 10.3x genome equivalents. The male BAC library was constructed using restriction enzyme EcoR I and consists of 55,296 clones with an average insert size of 101 kb, providing 15.0x genome equivalents. The male BAC library was used in constructing the physical map of the male-specific region of the male Y chromosome (MSY) and in filling gaps and extending the physical map of the hermaphrodite-specific region of the Yh chromosome (HSY) and the X chromosome physical map. The female BAC library was used to extend the X physical map gap. The MSY, HSY, and X physical maps offer a unique opportunity to study chromosomal rearrangements, Y chromosome degeneration, and dosage compensation of the papaya nascent sex chromosomes.
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影响因子：
5.4
作者：
Ming, R;Moore, PH;Paterson, AH
通讯作者：
Paterson, AH
影响因子：
7
作者：
Zhang, Wenli;Wang, Xiue;Jiang, Jiming
通讯作者：
Jiang, Jiming
影响因子：
6.2
作者：
Cheng, YH;Yang, JS;Yeh, SD
通讯作者：
Yeh, SD
影响因子：
7.2
作者：
Yu, Qingyi;Hou, Shaobin;Ming, Ray
通讯作者：
Ming, Ray
影响因子：
30.8
作者：
Mozo, T;Dewar, K;Altmann, T
通讯作者：
Altmann, T