Probiotic supplementation promotes calcification in Danio rerio larvae: a molecular study.
Probiotic supplementation promotes calcification in Danio rerio larvae: a molecular study.
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DOI：
10.1371/journal.pone.0083155
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Carnevali O
中科院分区：
文献类型：
--
作者：
Maradonna F;Gioacchini G;Falcinelli S;Bertotto D;Radaelli G;Olivotto I;Carnevali O
越来越多的研究表明，饮食益生菌可以对人类和动物产生有益的健康影响。我们以前证明，膳食补充剂与鼠李糖乳杆菌-一个组成部分的人类肠道微生物-增强繁殖，幼虫发育，和生物矿化过程中的斑马鱼（斑马鱼）。本研究的目的是确定L. rhamnosus在斑马鱼幼虫发育过程中。形态学和组织化学研究表明，L. rhamnosus通过刺激参与骨化的关键基因（例如，侏儒相关转录因子2（runx 2）、Sp 7转录因子（sp 7）、基质Gla蛋白（mgp）和骨γ-羧基谷氨酸（gla）蛋白（bglap））的表达以及通过抑制骨形成抑制剂sclerostin（sost））来加速骨沉积。对与成骨细胞和骨细胞分化有关的丝裂原活化蛋白激酶1和3-（Mapk 1和Mapk 3）进行Western印迹分析，证实了在受精后16天（dpf）Mapk 1和Mapk 3在接受L.补充鼠李糖。有趣的是，在同一个体中检测到的sost减少表明益生菌可能有助于治疗骨骼疾病。
A growing number of studies have been showing that dietary probiotics can exert beneficial health effects in both humans and animals. We previously demonstrated that dietary supplementation with Lactobacillus rhamnosus - a component of the human gut microflora - enhances reproduction, larval development, and the biomineralization process in Danio rerio (zebrafish). The aim of this study was to identify the pathways affected by L. rhamnosus during zebrafish larval development. Our morphological and histochemical findings show that L. rhamnosus accelerates bone deposition through stimulation of the expression of key genes involved in ossification, e.g. runt-related transcription factor 2 (runx2), Sp7 transcription factor (sp7), matrix Gla protein (mgp), and bone gamma-carboxyglutamate (gla) protein (bglap) as well as through inhibition of sclerostin (sost), a bone formation inhibitor. Western blot analysis of mitogen-activated protein kinase 1 and 3-(Mapk1 and Mapk3), which are involved in osteoblast and osteocyte differentiation, documented an increase in Mapk1 16 days post fertilization (dpf) and of Mapk3 23 dpf in individuals receiving L. rhamnosus supplementation. Interestingly, a reduction of sost detected in the same individuals suggests that the probiotic may help treat bone disorders.
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影响因子：
64.8
作者：
Ducy, P;Desbois, C;Karsenty, G
通讯作者：
Karsenty, G
影响因子：
64.5
作者：
Komori, T;Yagi, H;Kishimoto, T
通讯作者：
Kishimoto, T
影响因子：
3.8
作者：
Gioacchini, Giorgia;Maradonna, Francesca;Carnevali, Oliana
通讯作者：
Carnevali, Oliana
影响因子：
2.5
作者：
Flores, Maria Vega;Lam, Enid Yi Ni;Crosier, Kathryn
通讯作者：
Crosier, Kathryn
影响因子：
1.2
作者：
Flores, MV;Tsang, VWK;Fisher, S
通讯作者：
Fisher, S