Regulators of the protein phosphatase PP1γ2, PPP1R2, PPP1R7, and PPP1R11 are involved in epididymal sperm maturation.
Regulators of the protein phosphatase PP1γ2, PPP1R2, PPP1R7, and PPP1R11 are involved in epididymal sperm maturation.
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DOI：
10.1002/jcp.27130
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发表时间：
2019-03
影响因子：
5.6
通讯作者：
Vijayaraghavan S
中科院分区：
文献类型：
--
作者：
Goswami S;Korrodi-Gregório L;Sinha N;Bhutada S;Bhattacharjee R;Kline D;Vijayaraghavan S
丝氨酸/苏氨酸蛋白磷酸酶 1 (PP1) 抑制剂 PPP1R2、PPP1R7 和 PPP1R11 是进化上古老且高度保守的蛋白质。 PP1 存在四种亚型：PP1α、PP1β、PP1γ1 和 PP1γ2；除 PP1γ2 外，其中三个是普遍存在的。 PP1γ2 同工型仅存在于哺乳动物睾丸和精子中，这一事实导致人们认为精子中 PP1γ2 的同工型特异性调节因子可能负责其功能。在本报告中，我们研究了这些抑制剂 PPP1R2、R7 和 R11，以确定它们在睾丸中的空间和时间表达及其在精子中的调节功能。我们发现，与 PP1γ2 类似，这三种抑制剂在发育中的生精细胞中高水平表达。然而，与体细胞组织相比，调节因子的转录物在睾丸中表达为独特的大小。这三个调节因子与精子头部和主要部分的 PP1γ2 共享定位。我们发现抑制剂与 PP1γ2 的关联在附睾精子成熟过程中发生变化。在不动的附睾头精子中，PPP1R2 和 PPP1R7 不与 PP1γ2 结合，而在能动的尾部精子中，所有三种抑制剂均以异二聚体或异三聚体形式结合。在缺乏 sAC 和糖原合酶激酶 3 的雄性小鼠尾部精子中，运动性和生育能力受损，PP1γ2 与抑制剂的关联类似于未成熟的带头精子。由于磷酸化，调节因子与 PP1γ2 关联的变化是负责精子通过附睾期间运动和受精能力发展的生化机制的一部分。
The serine/threonine protein phosphatase 1 (PP1) inhibitors PPP1R2, PPP1R7, and PPP1R11 are evolutionarily ancient and highly conserved proteins. Four PP1 isoforms, PP1α, PP1β, PP1γ1, and PP1γ2, exist; three of them except PP1γ2 are ubiquitous. The fact that PP1γ2 isoform is present only in mammalian testis and sperm led to the notion that isoform-specific regulators for PP1γ2 in sperm may be responsible for its function. In this report, we studied these inhibitors, PPP1R2, R7, and R11, to determine their spatial and temporal expression in testis and their regulatory functions in sperm. We show that, similar to PP1γ2, the three inhibitors are expressed at high levels in developing spermatogenic cells. However, the transcripts for the regulators are expressed as unique sizes in testis compared with somatic tissues. The three regulators share localization with PP1γ2 in the head and the principal piece of sperm. We show that the association of inhibitors to PP1γ2 changes during epididymal sperm maturation. In immotile caput epididymal sperm, PPP1R2 and PPP1R7 are not bound to PP1γ2, whereas in motile caudal sperm, all three inhibitors are bound as heterodimers or heterotrimers. In caudal sperm from male mice lacking sAC and glycogen synthase kinase 3, where motility and fertility are impaired, the association of PP1γ2 to the inhibitors resembles immature caput sperm. Changes in the association of the regulators with PP1γ2, due to their phosphorylation, are part of biochemical mechanisms responsible for the development of motility and fertilizing ability of sperm during their passage through the epididymis.
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影响因子：
3.6
作者：
Bhattacharjee, Rahul;Goswami, Suranjana;Vijayaraghavan, Srinivasan
通讯作者：
Vijayaraghavan, Srinivasan
影响因子：
--
作者：
Korrodi-Gregório L;Ferreira M;Vintém AP;Wu W;Muller T;Marcus K;Vijayaraghavan S;Brautigan DL;da Cruz E Silva OA;Fardilha M;da Cruz E Silva EF
通讯作者：
da Cruz E Silva EF
DOI：
10.1046/j.1432-1327.1999.00344.x
发表时间：
1999-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Ceulemans, H;Van Eynde, A;Bollen, M
通讯作者：
Bollen, M
影响因子：
2.9
作者：
Chakrabarti, Rumela;Cheng, Lina;Vijayaraghavan, Srinivasan
通讯作者：
Vijayaraghavan, Srinivasan
影响因子：
3.7
作者：
Cheng L;Pilder S;Nairn AC;Ramdas S;Vijayaraghavan S
通讯作者：
Vijayaraghavan S