The Ubiquitin Conjugating Enzyme UbcD1 is Required for Notch Signaling Activation During Drosophila Wing Development.
The Ubiquitin Conjugating Enzyme UbcD1 is Required for Notch Signaling Activation During Drosophila Wing Development.
复制标题
果蝇翅膀发育过程中，Notch 信号激活需要泛素结合酶 UbcD1。
DOI：
10.3389/fgene.2021.770853
复制
发表时间：
2021
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhang F;Chen Y;Shen J;Zhang J
Notch信号通路在动物发育过程中起着重要作用。蛋白质泛素化通过控制主要信号组分的稳定性和活性而有助于Notch信号调节。在果蝇中的研究已经鉴定了多种修饰Notch配体和受体蛋白的泛素连接酶和去泛素化酶。泛素化底物的命运取决于所连接的泛素链的拓扑结构，这是由泛素缀合酶（E2酶）决定的。然而，哪些E2酶参与Notch信号转导仍然难以捉摸。在这里，我们报告说，E2酶UbcD1是必需的Notch信号激活在果蝇翅膀的发展。UbcD1的突变导致成虫翅的边缘切口和翅成虫盘中Notch信号传导靶表达的减少。遗传分析表明，UbcD1在Notch蛋白裂解之前在信号接收细胞中起作用。我们提供了进一步的证据表明，UbcD1可能参与Notch蛋白的内吞运输。我们的研究结果表明，UbcD1正调控Notch信号转导，从而揭示了UbcD1在发育中的新作用。
Notch signaling pathway plays crucial roles in animal development. Protein ubiquitination contributes to Notch signaling regulation by governing the stability and activity of major signaling components. Studies in Drosophila have identified multiple ubiquitin ligases and deubiquitinating enzymes that modify Notch ligand and receptor proteins. The fate of ubiquitinated substrates depend on topologies of the attached ubiquitin chains, which are determined by the ubiquitin conjugating enzymes (E2 enzymes). However, which E2 enzymes participate in Notch signal transduction remain elusive. Here, we report that the E2 enzyme UbcD1 is required for Notch signaling activation during Drosophila wing development. Mutations of UbcD1 lead to marginal nicks in the adult wing and reduction of Notch signaling targets expression in the wing imaginal disc. Genetic analysis reveal that UbcD1 functions in the signaling receiving cells prior to cleavage of the Notch protein. We provide further evidence suggesting that UbcD1 is likely involved in endocytic trafficking of Notch protein. Our results demonstrate that UbcD1 positively regulates Notch signaling and thus reveal a novel role of UbcD1 in development.
登录
查看更多内容
影响因子：
10.5
作者：
Cenci, G;Rawson, RB;Gatti, M
通讯作者：
Gatti, M
影响因子：
4.8
作者：
Chen, Li;Paquette, Nicholas;Silverman, Neal
通讯作者：
Silverman, Neal
影响因子：
11.8
作者：
Itoh, M;Kim, CH;Chitnis, AB
通讯作者：
Chitnis, AB
影响因子：
5.8
作者：
Irles P;Elshaer N;Piulachs MD
通讯作者：
Piulachs MD
影响因子：
4.6
作者：
Hori, K;Fostier, M;Matsuno, K
通讯作者：
Matsuno, K