E3 ubiquitin ligase SOR1 regulates ethylene response in rice root by modulating stability of Aux/IAA protein.
E3 ubiquitin ligase SOR1 regulates ethylene response in rice root by modulating stability of Aux/IAA protein.
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E3泛素连接酶SOR1通过调节Aux/IAA蛋白的稳定性来调节水稻根部的乙烯反应
DOI：
10.1073/pnas.1719387115
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发表时间：
2018-04-24
影响因子：
11.1
通讯作者：
Zhang JS
中科院分区：
文献类型：
--
作者：
Chen H;Ma B;Zhou Y;He SJ;Tang SY;Lu X;Xie Q;Chen SY;Zhang JS
生长素信号组分参与乙烯介导的根伸长抑制。然而，TIR 1/AFB 2-auxin-Aux/indole acetic acid（IAA）信号转导与乙烯反应之间的相互作用仍有待于详细阐明。在这项研究中，我们报告了E3泛素连接酶土壤表面生根1（SOR 1），它的目标是一个非经典的Aux/IAA蛋白OsIAA 26的26 S蛋白酶体介导的降解。SOR 1的E3连接酶活性可被典型的Aux/IAA蛋白OsIAA 9抑制，OsIAA 9是OsTIR 1/AFB 2的靶蛋白。我们的研究确定了一个潜在的调节生长素介导的乙烯反应在生长素信号水平。植物激素乙烯和生长素协同调节植物根的生长和发育。泛素介导的E3泛素连接酶SCFTIR 1/AFB对Aux/IAA转录抑制子的蛋白水解触发了基于转录的生长素信号传导。在这里，我们表明，水稻（Oryza sativa L.）土壤表面生根1（SOR 1）是从水稻乙烯不敏感突变体mHZ 2/SOR 1 -2中鉴定的环指E3泛素连接酶，其通过调节Aux/IAA蛋白的稳定性来控制根特异性乙烯反应。SOR 1与OsIAA 26和OsIAA 9发生物理相互作用，OsIAA 26和OsIAA 9分别是非典型和典型的Aux/IAA蛋白。SOR 1靶向OsIAA 26进行泛素/26 S蛋白酶体介导的降解，而OsIAA 9通过抑制SOR 1的E3活性来保护OsIAA 26蛋白免于降解。生长素通过促进SCFOsTIR 1/AFB 2介导和SOR 1辅助的OsIAA 9蛋白的去稳定化来促进SOR 1依赖的OsIAA 26降解。我们的研究提供了一个候选机制，SOR 1-OsIAA 26模块作用于OsTIR 1/AFB 2-auxin-OsIAA 9信号转导的下游，调节乙烯抑制水稻幼苗根系生长。
Significance Auxin signaling components participate in ethylene-mediated inhibition of root elongation. However, the interplay between TIR1/AFB2-auxin-Aux/indole acetic acid (IAA) signaling and ethylene response remains to be elucidated in detail. In this study, we report an E3 ubiquitin ligase soil-surface rooting 1 (SOR1), which targets a noncanonical Aux/IAA protein OsIAA26 for 26S proteasome-mediated degradation. The E3 ligase activity of SOR1 can be repressed by the canonical Aux/IAA protein OsIAA9, which is the target of OsTIR1/AFB2. Our study identifies a potential regulator that modulates auxin-mediated ethylene response at the auxin signaling level. Plant hormones ethylene and auxin synergistically regulate plant root growth and development. Ubiquitin-mediated proteolysis of Aux/IAA transcriptional repressors by the E3 ubiquitin ligase SCFTIR1/AFB triggers a transcription-based auxin signaling. Here we show that rice (Oryza sativa L.) soil-surface rooting 1 (SOR1), which is a RING finger E3 ubiquitin ligase identified from analysis of a rice ethylene-insensitive mutant mhz2/sor1-2, controls root-specific ethylene responses by modulating Aux/IAA protein stability. SOR1 physically interacts with OsIAA26 and OsIAA9, which are atypical and canonical Aux/IAA proteins, respectively. SOR1 targets OsIAA26 for ubiquitin/26S proteasome-mediated degradation, whereas OsIAA9 protects the OsIAA26 protein from degradation by inhibiting the E3 activity of SOR1. Auxin promotes SOR1-dependent degradation of OsIAA26 by facilitating SCFOsTIR1/AFB2-mediated and SOR1-assisted destabilization of OsIAA9 protein. Our study provides a candidate mechanism by which the SOR1-OsIAA26 module acts downstream of the OsTIR1/AFB2-auxin-OsIAA9 signaling to modulate ethylene inhibition of root growth in rice seedlings.
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影响因子：
4.9
作者：
Rahman, A;Amakawa, T;Tsurumi, S
通讯作者：
Tsurumi, S
DOI：
10.1073/pnas.1014856107
发表时间：
2010-12-14
影响因子：
11.1
作者：
Carranco, Raul;Manuel Espinosa, Jose;Jordano, Juan
通讯作者：
Jordano, Juan
影响因子：
64.8
作者：
Osterlund, MT;Hardtke, CS;Deng, XW
通讯作者：
Deng, XW
影响因子：
64.5
作者：
Potuschak, T;Lechner, E;Genschik, P
通讯作者：
Genschik, P
DOI：
10.1073/pnas.0401698101
发表时间：
2004-04-27
影响因子：
11.1
作者：
Gagne, JM;Smalle, J;Vierstra, RD
通讯作者：
Vierstra, RD