QTL Mapping in Three Connected Populations Reveals a Set of Consensus Genomic Regions for Low Temperature Germination Ability in Zea mays L.
QTL Mapping in Three Connected Populations Reveals a Set of Consensus Genomic Regions for Low Temperature Germination Ability in Zea mays L.
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三个相关群体的 QTL 作图揭示了玉米低温萌发能力的一组共有基因组区域。
DOI：
10.3389/fpls.2018.00065
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发表时间：
2018
影响因子：
5.6
通讯作者：
Gu R
中科院分区：
文献类型：
--
作者：
Li X;Wang G;Fu J;Li L;Jia G;Ren L;Lubberstedt T;Wang G;Wang J;Gu R
提高种子活力以应对低温胁迫是玉米育种的一个重要目标，可以使玉米提前播种。以两个耐寒自交系220和P9-10和两个感病自交系Y1518和PH4CV为材料，构建了3个与种子低温发芽力相关的数量性状基因座(QTL)。在10℃下，从沙床上采集了两个发芽性状(出苗率和发芽指数)，从纸卷上提取了三个苗木性状(苗根长、地上部长和全长)。在所有群体中，所有性状之间存在显著的相关性。通过单群体分析，共检测到43个QTL，解释表型变异为0.62%∼39.44%。17个QTL解释了10%以上的表型变异，其中16个QTL(94.12%)遗传了耐性品系的有利等位基因。构建共识图谱后，确定了3个元QTL(MQTL)，它们至少包含两个来自不同群体的初始QTL。MQTL1-1包括7个控制发芽和幼苗性状的初始QTL，其中3个解释了30%以上的表型变异。MQTL2-1和mQTL9-1包含2-3个初始QTL。这三个mQTL区域的QTL的有利等位基因均遗传自耐受系220和P9-10。这些结果为克隆玉米萌发过程中mQTL区域的基因，揭示玉米萌发耐冷性的分子机制奠定了基础。
Improving seed vigor in response to cold stress is an important breeding objective in maize that allows early sowing. Using two cold tolerant inbred lines 220 and P9-10 and two susceptible lines Y1518 and PH4CV, three connected F2:3 populations were generated for detecting quantitative trait locus (QTL) related to seed low-temperature germination ability. At 10°C, two germination traits (emergence rate and germination index) were collected from a sand bed and three seedling traits (seedling root length, shoot length, and total length) were extracted from paper rolls. Significant correlations were found among all traits in all populations. Via single-population analysis, 43 QTL were detected with explained phenotypic variance of 0.62%∼39.44%. Seventeen QTL explained more than 10% phenotypic variance; of them sixteen (94.12%) inherited favorable alleles from the tolerant lines. After constructing a consensus map, three meta-QTL (mQTL) were identified to include at least two initial QTL from different populations. mQTL1-1 included seven initial QTL for both germination and seedling traits; with three explaining more than 30% phenotypic variance. mQTL2-1 and mQTL9-1 covered two to three initial QTL. The favorable alleles of the QTL within these three mQTL regions were all inherited from the tolerant line 220 and P9-10. These results provided a basis for cloning of genes underlying the mQTL regions to uncover the molecular mechanisms of maize cold tolerance during germination.
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DOI：
10.1073/pnas.0805303105
发表时间：
2008-08-26
影响因子：
11.1
作者：
Fujino, Kenji;Sekiguchi, Hiroshi;Yano, Masahiro
通讯作者：
Yano, Masahiro
玉米 IBM Syn4 RIL 群体低温发芽能力的 QTL 定位
影响因子：
3.7
作者：
Hu S;Lübberstedt T;Zhao G;Lee M
通讯作者：
Lee M
影响因子：
5.4
作者：
Fujino, K;Sekiguchi, H;Yano, M
通讯作者：
Yano, M
影响因子：
3.7
作者：
Han Z;Ku L;Zhang Z;Zhang J;Guo S;Liu H;Zhao R;Ren Z;Zhang L;Su H;Dong L;Chen Y
通讯作者：
Chen Y
影响因子：
6.9
作者：
Jompuk, C;Fracheboud, Y;Leipner, J
通讯作者：
Leipner, J