Schwannomin/merlin promotes Schwann cell elongation and influences myelin segment length.
Schwannomin/merlin promotes Schwann cell elongation and influences myelin segment length.
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DOI：
10.1016/j.mcn.2010.12.006
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发表时间：
2011-05
影响因子：
3.5
通讯作者：
Fernandez-Valle, Cristina
中科院分区：
文献类型：
--
作者：
Thaxton, Courtney;Bott, Marga;Walker, Barbara;Sparrow, Nicklaus A.;Lambert, Stephen;Fernandez-Valle, Cristina
神经纤维瘤病2型肿瘤抑制因子，神经鞘蛋白（schwannomin，Sch）是一种调节受体信号和肌动蛋白动力学的质膜-细胞骨架连接蛋白。我们研究了雪的作用，在指定的形态学变化所需的雪旺细胞（SC）的功能在体外。分离的Sch-GFP表达的SC延长双极过程比GFP表达细胞形成的过程长82%。相比之下，表达显性阴性Sch-BBA-GFP的SC延长双极过程比对照短16%，比表达Sch-GFP的SC短64%。nf 2基因失活导致分离的小鼠SC从双极细胞向多极细胞转变。实时成像显示，SC共表达Sch-GFP和显性阴性RacN 17表现出类似的背根神经节外植体培养物，他们迅速对齐轴突和缓慢延长双极过程。相反，表达组成型活性RacV 12的SC在形态上经历了连续的转变，这干扰了轴突排列。当与神经元在髓鞘促进条件下共培养时，Sch-GFP表达的SC比GFP表达的SC产生更长的髓鞘段。相比之下，表达Sch-BBA-GFP的SC未能在轴突上对齐或形成髓鞘。总之，这些结果表明，Sch在诱导和/或维持SC的纺锤体形状中起着至关重要的作用，并表明该机制涉及Sch依赖性抑制Rac活性。通过稳定双极形态，Sch促进SC与轴突对齐，并最终影响髓鞘段长度。
The Neurofibromatosis type 2 tumor suppressor, schwannomin (Sch) is a plasma membrane-cytoskeleton linking protein that regulates receptor signaling and actin dynamics. We examined Sch’s role in specifying morphological changes needed for Schwann cell (SC) function in vitro. Isolated Sch-GFP-expressing SCs extended bipolar processes 82% longer than those formed by GFP-expressing cells. In contrast, SCs expressing dominant negative Sch-BBA-GFP extended bipolar processes 16% shorter than controls and 64% shorter than Sch-GFP-expressing SCs. nf2 gene inactivation caused isolated mouse SCs to transition from bipolar to multipolar cells. Live imaging revealed that SCs co-expressing Sch-GFP and dominant negative RacN17 behaved similarly in dorsal root ganglion explant cultures; they quickly aligned on axons and slowly elongated bipolar processes. In contrast, SCs expressing constitutively active RacV12 underwent continuous transitions in morphology that interfered with axon alignment. When co-cultured with neurons under myelin-promoting conditions, Sch-GFP-expressing SCs elaborated longer myelin segments than GFP-expressing SCs. In contrast, Sch-BBA-GFP-expressing SCs failed to align on or myelinate axons. Together, these results demonstrate that Sch plays an essential role in inducing and/or maintaining the SC’s spindle shape and suggest that the mechanism involves Sch-dependent inhibition of Rac activity. By stabilizing the bipolar morphology, Sch promotes alignment of SCs with axons and ultimately influences myelin segment length.
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影响因子：
7.8
作者：
Einheber, S;Zanazzi, G;Ching, W;Scherer, S;Milner, TA;Peles, E;Salzer, JL
通讯作者：
Salzer, JL
DOI：
10.1083/jcb.200503165
发表时间：
2005-10-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Okada T;Lopez-Lago M;Giancotti FG
通讯作者：
Giancotti FG
影响因子：
4.8
作者：
Alfthan, K;Heiska, L;Carpen, O
通讯作者：
Carpen, O
影响因子：
4.8
作者：
Kissil, JL;Johnson, KC;Jacks, T
通讯作者：
Jacks, T
影响因子：
3
作者：
Pannunzio, ME;Jou, IM;Balian, G
通讯作者：
Balian, G