Proteomic analysis of laser microdissected melanoma cells from skin organ cultures.
Proteomic analysis of laser microdissected melanoma cells from skin organ cultures.
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DOI：
10.1021/pr100164x
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发表时间：
2010-07-02
影响因子：
4.4
通讯作者：
Conrads TP
中科院分区：
文献类型：
--
作者：
Hood BL;Grahovac J;Flint MS;Sun M;Charro N;Becker D;Wells A;Conrads TP
深入了解控制从原位黑色素瘤到晚期黑色素瘤进展的分子事件，并了解黑色素瘤部位的局部微环境如何影响这种进展，是迄今为止很大程度上尚未探索的两个临床关键方面。为了确定黑色素瘤细胞与黑色素瘤皮肤微环境之间串扰的关键调节因子，将原发性和转移性人类黑色素瘤细胞接种到皮肤器官培养物（SOC）中，并生长两周。通过激光显微切割从 SOC 中回收黑色素瘤细胞，并使用 LTQ-Orbitrap 进行纳流液相色谱-串联质谱分析全细胞胰蛋白酶消化物。通过光谱计数计算差异蛋白质丰度，其结果提供了证据，证明在这些黑色素瘤细胞存在的情况下细胞基质和细胞粘附分子上调，概括了通过对侵袭性黑色素瘤的人体活检和邻近非受累皮肤的组织样本进行比较分析获得的蛋白质组数据。这一一致性证明了 SOC 在黑色素瘤微环境蛋白质组学研究中的价值。
Gaining insights into the molecular events that govern the progression from melanoma in situ to advanced melanoma, and understanding how the local microenvironment at the melanoma site influences this progression, are two clinically pivotal aspects that to date are largely unexplored. In an effort to identify key regulators of the crosstalk between melanoma cells and the melanoma-skin microenvironment, primary and metastatic human melanoma cells were seeded into skin organ cultures (SOCs), and grown for two weeks. Melanoma cells were recovered from SOCs by laser microdissection and whole-cell tryptic digests analyzed by nanoflow liquid chromatography-tandem mass spectrometry with an LTQ-Orbitrap. The differential protein abundances were calculated by spectral counting, the results of which provides evidence that cell-matrix and cell-adhesion molecules that are upregulated in the presence of these melanoma cells recapitulate proteomic data obtained from comparative analysis of human biopsies of invasive melanoma and a tissue sample of adjacent, non-involved skin. This concordance demonstrates the value of SOCs for conducting proteomic investigations of the melanoma microenvironment.
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影响因子：
3.2
作者：
Bausch, Dirk;Mino-Kenudson, Mari;Fernandez-del Castillo, Carlos;Warshaw, Andrew L.;Kelly, Kimberly A.;Thayer, Sarah P.
通讯作者：
Thayer, Sarah P.
影响因子：
15.9
作者：
Bornstein, P
通讯作者：
Bornstein, P
影响因子：
5.6
作者：
Iyer, Anand Krishnan V.;Tran, Kien T.;Wells, Alan
通讯作者：
Wells, Alan
DOI：
10.1016/j.bbapap.2008.09.023
发表时间：
2009-02-01
影响因子：
3.2
作者：
Nawarak, Jiraporn;Huang-Liu, Rosa;Cheng, Sun-Long
通讯作者：
Cheng, Sun-Long
影响因子：
4.1
作者：
Bornstein, Paul
通讯作者：
Bornstein, Paul