Adaptive dif Modules in Permafrost Strains of Acinetobacter lwoffii and Their Distribution and Abundance Among Present Day Acinetobacter Strains
Adaptive dif Modules in Permafrost Strains of Acinetobacter lwoffii and Their Distribution and Abundance Among Present Day Acinetobacter Strains
复制标题
鲁氏不动杆菌永久冻土菌株中的适应性 diff 模块及其在当今不动杆菌菌株中的分布和丰度
DOI：
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发表时间：
2019
影响因子：
5.2
通讯作者：
M. Petrova
中科院分区：
文献类型：
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作者：
S. Mindlin;A. Beletsky;A. Mardanov;M. Petrova
位点特异性重组的dif/Xer系统允许在细菌DNA复制期间解析染色体二聚体。最近，它也被证明参与了一些已知的Xer依赖的移动的元素的水平转移。在这里，我们表明，质粒的各种不动杆菌属物种，包括临床上重要的菌株，往往含有多个pdif网站，主要位于其附件区域。不动杆菌属菌株的染色体也可能含有额外的dif位点，并且它们与质粒pdif位点的相似性高于与主要染色体位点dif 1的相似性。我们进一步确定了假定的移动的遗传元件包含适应基因两侧的pdif位点，并分析了它们在不动杆菌属物种中的分布。总之，我们描述了7个移动的元素，包含具有各种适应功能的基因，从冻土株的A。lwoffii集团。所有这些基因也在包括A.鲍曼不动杆菌。我们不能检测到pdif网站和相应的移动的元素密切相关的细菌属，包括嗜冷杆菌和莫拉氏菌。因此，不同模块的广泛分布是不动杆菌属物种的特征，并且可能有助于它们在环境和临床中的高适应性。
The dif/Xer system of site-specific recombination allows resolution of chromosomal dimers during bacterial DNA replication. Recently, it was also shown to be involved in horizontal transfer of a few known Xer-dependent mobile elements. Here, we show that plasmids of various Acinetobacter species, including clinically important strains, often contain multiple pdif sites that are mainly located within their accessory regions. Chromosomes of Acinetobacter strains may also contain additional dif sites, and their similarity with plasmid pdif sites is higher than with the main chromosomal site dif1. We further identify putative mobile genetic elements containing pdif sites on both flanks of adaptive genes and analyze their distribution in Acinetobacter species. In total, we describe seven mobile elements containing genes with various adaptive functions from permafrost strains of A. lwoffii group. All of them are also spread in modern plasmids of different Acinetobacter species including A. baumannii. We could not detect pdif sites and corresponding mobile elements in closely related bacterial genera, including Psychrobacter and Moraxella. Thus, the widespread distribution of dif modules is a characteristic feature of Acinetobacter species and may contribute to their high adaptability both in the environment and in the clinic.
影响因子：
14.9
作者：
Altschul, SF;Madden, TL;Lipman, DJ
通讯作者：
Lipman, DJ
影响因子：
7
作者：
Crooks, GE;Hon, G;Brenner, SE
通讯作者：
Brenner, SE