Down-modulation of neutrophil production by erythropoietin in human hematopoietic clones.
Down-modulation of neutrophil production by erythropoietin in human hematopoietic clones.
复制标题
人类造血克隆中促红细胞生成素下调中性粒细胞的产生。
作者：
R. Christensen;JM Koenig;D. Viskochil;G. Rothstein
在造血祖细胞的克隆形成试验中，高浓度（4 U/mL）的促红细胞生成素（epo）减少了粒细胞-巨噬细胞（GM）集落的形成，并减少了每个培养板形成的粒细胞数量。胎儿祖细胞对epo的这些作用比成人祖细胞更敏感，在大于或等于1 U epo/mL时显示这些减少。通过在不存在EPO的情况下生长由重组GM-CSF刺激的胎儿祖细胞来研究该机制，并且当八细胞克隆首次出现时，绘制它们的位置，然后添加EPO，并评估其对克隆随后分化的影响。在没有epo的情况下，克隆只发育成GM菌落。然而，如果发育中的克隆与epo，85%成熟为GM集落，但15%成为多系或normoblasts集落。此外，发育中的克隆在epo的作用下产生的菌落中含有较少的中性粒细胞。在三种重组epo和纯化的人epo中均观察到epo对中性粒细胞生成的这些影响，但在用兔抗epo抗血清中和的epo中未观察到。
In clonogenic assays of hematopoietic progenitors, high concentrations (4 U/mL) of erythropoietin (epo) reduced the formation of granulocyte-macrophage (GM) colonies and diminished the number of granulocytes formed per culture plate. Fetal progenitors were more sensitive to these effects of epo than were progenitors from adults, displaying these reductions at greater than or equal to 1 U epo/mL. The mechanism was investigated by growing fetal progenitors stimulated by recombinant GM-CSF, in the absence of epo, and when eight-cell clones first appeared, mapping their location, then adding epo, and assessing its effect on the subsequent differentiation of the clones. In the absence of epo, the clones developed exclusively into GM colonies. However, if developing clones were presented with epo, 85% matured into GM colonies, but 15% became multilineage or normoblast colonies. In addition, developing clones that were presented with epo produced colonies that contained fewer neutrophils. These effects of epo on neutrophil generation were observed with each of three varieties of recombinant epo, and also with purified human epo, but were not observed using epo that had been neutralized with rabbit anti-epo antiserum.
重组人造血生长因子靶标的无血清培养分析：白细胞介素 3 和粒细胞/巨噬细胞集落刺激因子对早期发育阶段具有特异性。
DOI：
10.1073/pnas.85.12.4360
发表时间：
1988
影响因子：
11.1
作者：
Sonoda,Y;Yang,YC;Wong,GG;Clark,SC;Ogawa,M
通讯作者：
Ogawa,M
重组长臂猿白细胞介素 3 支持培养中人多谱系集落和母细胞集落的形成：与重组人粒细胞-巨噬细胞集落刺激因子的比较。
影响因子：
20.3
作者：
Leary,AG;Yang,YC;Clark,SC;Gasson,JC;Golde,DW;Ogawa,M
通讯作者：
Ogawa,M