Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
复制标题
DOI:
10.1371/journal.pone.0041028
复制
发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Prescott M
中科院分区:
文献类型:
--
作者:
Pettikiriarachchi A;Gong L;Perugini MA;Devenish RJ;Prescott M
We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε 585 nm, 64,000 M−1. cm−1) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
登录
查看更多内容
影响因子:
3.4
作者:
Schuck, P
通讯作者:
Schuck, P
影响因子:
8
作者:
Higgins, Gavin C.;Beart, Philip M.;Nagley, Phillip
通讯作者:
Nagley, Phillip
影响因子:
48
作者:
Ai, Hui-wang;Hazelwood, Kristin L.;Campbell, Robert E.
通讯作者:
Campbell, Robert E.
影响因子:
2.6
作者:
Sheff, MA;Thorn, KS
通讯作者:
Thorn, KS
影响因子:
3.5
作者:
Gurskaya, NG;Fradkov, AF;Lukyanov, SA
通讯作者:
Lukyanov, SA