Continuous-flow analysis of proteoglycans during gel filtration using guanidine hydrochloride as an eluent.
Continuous-flow analysis of proteoglycans during gel filtration using guanidine hydrochloride as an eluent.
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使用盐酸胍作为洗脱液在凝胶过滤过程中对蛋白聚糖进行连续流动分析。
DOI：
10.1016/s0021-9673(01)88248-0
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Berenson,GS
中科院分区：
文献类型：
--
作者：
Radhakrishnamurthy,B;Jeansonne,N;Berenson,GS
Gel filtration is one of the procedures used for the study of molecular sizes of proteoglycan subunits and aggregates’. The technique has also been used for the preliminary fractionation and purification of proteoglycans from crude extracts of tissues by 4.0 M guanidine hydrochloride2T3. Fractions of gel filtration are monitored for proteoglycans usually by uranic acid analysis from a carbazole reaction4-‘. As salts interfere in the analysis, the fractions have to be dialyzed exhaustively against distilled water or a low ionic concentration of sodium acetate prior to analysis. The dialysis of fractions and manual analysis of uranic acid are time consuming. This report describes a procedure using an orcinol-sulfuric acid reaction in the Technicon sugar chromatography system. A continuous-flow analysis of proteoglycans during elution by guanidine hydrochloride solutions from gel filtration columns was developed, and its application to studies of proteoglycans under associative and dissociative conditions is described.
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DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Salisbury,BG;Wagner,WD
通讯作者：
Wagner,WD
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